Neuronal location of 5-hydroxytryptamine3 receptor-like immunoreactivity in the rat colon
Neuronal location of 5-hydroxytryptamine3 receptor-like immunoreactivity in the rat colon
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DOI：
10.1016/s0306-4522(02)00775-3
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发表时间：
2003-01-01
期刊：
影响因子：
3.3
通讯作者：
Clerc, N
中科院分区：
文献类型：
--
作者：
Mazzia, C;Hicks, GA;Clerc, N
肠神经元上存在5-羟色胺（3）受体，这是从40多年前的药理学数据中得知的。然而，由于没有合适的抗血清，还没有对哪些神经元具有这些受体进行充分的说明。我们已经发现，大多数针对5-羟色胺（3）受体序列的抗血清也识别前生长抑素原。我们报告说，这种来源的错误标记可以消除预孵育的抗血清与肽设计用于此目的。我们用预先吸收的抗血清定位大鼠结肠中的5-羟色胺（3）受体。免疫反应性神经细胞体发生在肌间神经节和粘膜下神经节。大多数细胞体光滑，突起长而光滑，即Dogiel II型形态。Dogiel II型神经元长突起的起始段呈强免疫反应性。约12%的免疫反应阳性肌间神经细胞大小相同或更小，并有多个短丝状突起。一些免疫反应的Dogiel II型神经元也在两个神经丛的calretinin免疫反应，和大多数粘膜下神经节的calbindin免疫反应。特异性免疫反应性发生在非静脉曲张的肌间神经节和粘膜下神经节中的纤维以及穿过纵向和环形肌肉层的纤维束中，但不发生在静脉曲张的肌间神经节和粘膜下神经节中。仅在粘膜中观察到免疫反应性曲张纤维。结论：5-羟色胺（3）受体存在于大鼠结肠的内在感觉神经元和支配结肠的外在感觉神经纤维上。(C)2003年IBRO。由爱思唯尔科技有限公司出版。保留所有权利。
The presence of 5-hydroxytryptamine(3) receptors on enteric neurons is known from pharmacological data that date back more than 40 years. However, an adequate account of which neurons bear these receptors has not been made because suitable antisera have not been available. We have found that the majority of antisera that have been raised against sequences from the 5-hydroxytryptamine(3) receptor also recognize pre-prosomatostatin. We report that this source of false labeling can be eliminated by pre-incubating the antisera with a peptide designed for this purpose. We have used the pre-absorbed antiserum to localize 5-hydroxytryptamine(3) receptors in the rat colon. Immunoreactive nerve cell bodies occurred in the myenteric and submucosal ganglia. The majority had smooth cell bodies and long, smooth processes, that is, Dogiel type II morphology. The initial segments of the long processes of the Dogiel type II neurons were strongly immunoreactive. About 12% of immunoreactive myenteric nerve cells were of the same or smaller size, and had multiple short filamentous processes. Some of the immunoreactive Dogiel type II neurons were also immunoreactive for calretinin in both plexuses, and the majority were immunoreactive for calbindin in submucosal ganglia. Specific immunoreactivity occurred in non-varicose, but not in varicose, fibers in the myenteric and submucosal ganglia, and in fiber bundles that traversed the longitudinal and circular muscle layers. Immunoreactive varicose fibers were observed only in the mucosa. It is concluded that 5-hydroxytryptamine(3) receptors occur on intrinsic sensory neurons in the rat colon, and on extrinsic sensory nerve fibers that innervate the colon. (C) 2003 IBRO. Published by Elsevier Science Ltd. All rights reserved.