Regulation of asialoglycoprotein receptor expression in rat hepatocytes cultured under proliferative conditions.
Regulation of asialoglycoprotein receptor expression in rat hepatocytes cultured under proliferative conditions.
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增殖条件下培养的大鼠肝细胞脱唾液酸糖蛋白受体表达的调节。
DOI：
10.1006/excr.1994.1018
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发表时间：
1994
影响因子：
3.7
通讯作者：
L. Dini
中科院分区：
文献类型：
--
作者：
L. Conti Devirgiliis;M. Massimi;G. Bruscalupi;A. Felici;L. Dini
肝细胞表面许多糖蛋白的表达与肝脏的分化状态有关。特别是，去唾液酸糖蛋白受体(ASGP-R)在许多生理和病理条件下被调节，在这些条件下肝细胞的增殖活性被改变。我们研究了加入表皮生长因子和胰岛素刺激大鼠肝细胞增殖的ASGP-R在原代单层培养中的表达。在增殖的肝细胞中，细胞表面的受体浓度降低，其分布没有改变；同样，结合活性和特异性转录本的数量都降低了。这种下降与细胞生长的高峰有关，通过[~3H]胸腺嘧啶核苷掺入DNA来判断。这些结果表明，在体外系统中也存在细胞周期依赖的ASGP-R表达；这种控制可能是由于受体特异性mRNA分子的可获得性降低或转录本的稳定性造成的。
The expression of many glycoproteins on the surface of hepatocytes has been related to the state of differentiation of the liver. In particular, the asialoglycoprotein receptor (ASGP-R) is modulated in many physiological and pathological conditions in which hepatocyte proliferative activity is modified. We studied ASGP-R expression in primary monolayer cultures of rat hepatocytes stimulated to proliferate by the addition of epidermal growth factor and insulin. In proliferating hepatocytes the receptor concentration on the cell surface was lowered, with no modifications in its distribution; similarly, both the binding activity and the amount of specific transcripts were decreased. The decreases were related to the peak of cellular growth, as judged by [3H]thymidine incorporation into DNA. The results suggest the presence of a cell cycle-dependent ASGP-R expression also in in vitro systems; this control could be exerted by a decreased availability of receptor-specific mRNA molecules or on the stability of transcripts.