Lutispora saccharofermentans sp. nov., a mesophilic, non-spore-forming bacterium isolated from a lab-scale methanogenic landfill bioreactor digesting anaerobic sludge, and emendation of the genus Lutispora to include species which are non-spore-forming and mesophilic.
Lutispora saccharofermentans sp. nov., a mesophilic, non-spore-forming bacterium isolated from a lab-scale methanogenic landfill bioreactor digesting anaerobic sludge, and emendation of the genus Lutispora to include species which are non-spore-forming and mesophilic.
复制标题
DOI：
10.1099/ijsem.0.005683
复制
发表时间：
2023-01
影响因子：
2.8
通讯作者：
A. El Houari;Morgan G Carpenter;Daniel Chaplin;P. Golyshin;J. McDonald
中科院分区：
文献类型：
--
作者：
A. El Houari;Morgan G Carpenter;Daniel Chaplin;P. Golyshin;J. McDonald
从含有厌氧消化池污泥的实验室规模产甲烷垃圾填埋场生物反应器中分离到一株新型厌氧、中温、无芽胞的产甲烷富集菌(菌株m25T)。细胞革兰氏染色阴性，过氧化氢酶阳性，氧化物酶阴性，杆状，靠鞭毛活动。基因组DNA G+C含量为40.11摩尔%。该菌生长的最适氯化钠浓度为2.5g L-1，最适温度为35℃，最适pH值为7.0。菌株m25T能在甘油、丙酮酸、精氨酸、半胱氨酸等不含酵母膏的条件下生长。在0.2%酵母膏存在下，菌株m25T以碳水化合物为基础生长，能够利用葡萄糖、纤维二糖、果糖、棉籽糖和半乳糖。该新菌株能利用甘油、尿素、丙酮酸、蛋白胨和胰蛋白胨。主要脂肪酸为iso-C15：0、C14：0、C16：0DMA和iso-C15：0DMA。基于16S rRNA基因序列的系统发育分析表明，该新菌株与嗜热黄斑病菌EBR46T亲缘关系较近，16S rRNA基因序列相似性达95.02%。通过平均核苷酸同源性和氨基酸同源性分析确定了菌株m25T的基因组亲缘关系，这两个结果都有力地支持了菌株m25T属于黄粉菌属。根据其独特的系统发育特征，菌株m25T被认为是黄霉属的一个新种。此外，根据其独特的生理特性，主要是缺乏孢子形成，还提出了修改黄粉菌属的建议，将非产孢型和中嗜性种包括在内。发酵型黄粉菌11月是提议的。该种的模式菌株为m25T(=DSM 112749T=ATCC TSD-268T)。
A novel anaerobic, mesophilic, non-spore-forming bacterium (strain m25T) was isolated from methanogenic enrichment cultures obtained from a lab-scale methanogenic landfill bioreactor containing anaerobic digester sludge. Cells were Gram-stain-negative, catalase-positive, oxidase-negative, rod-shaped, and motile by means of a flagellum. The genomic DNA G+C content was 40.11 mol%. The optimal NaCl concentration, temperature and pH for growth were 2.5 g l-1, 35 °C and at pH 7.0, respectively. Strain m25T was able to grow in the absence of yeast extract on glycerol, pyruvate, arginine and cysteine. In the presence of 0.2 % yeast extract, strain m25T grew on carbohydrates and was able to use glucose, cellobiose, fructose, raffinose and galactose. The novel strain could utilize glycerol, urea, pyruvate, peptone and tryptone. The major fatty acids were iso-C15 : 0, C14 : 0, C16 : 0 DMA (dimethyl acetal) and iso-C15 : 0 DMA. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the new isolate was closely related to Lutispora thermophila EBR46T (95.02 % 16S rRNA gene sequence similarity). Genome relatedness was determined using both average nucleotide identity and amino acid identity analyses, the results of which both strongly supported that strain m25T belongs to the genus Lutispora. Based on its unique phylogenetic features, strain m25T is considered to represent a novel species within the genus Lutispora. Moreover, based on its unique physiologic features, mainly the lack of spore formation, a proposal to amend the genus Lutispora is also provided to include the non-spore-forming and mesophilic species. Lutispora saccharofermentans sp. nov. is proposed. The type strain of the species is m25T (=DSM 112749T=ATCC TSD-268T).