Comparisons of Porcine Hepatocyte Spheroids and Single Hepatocytes in the Non-Woven Fabric Bioartificial Liver Module
Comparisons of Porcine Hepatocyte Spheroids and Single Hepatocytes in the Non-Woven Fabric Bioartificial Liver Module
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无纺布生物人工肝模块中猪肝细胞球体与单个肝细胞的比较
DOI：
10.1177/039139889601901008
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发表时间：
1996
期刊：
影响因子：
--
通讯作者：
T. Muto
中科院分区：
文献类型：
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作者：
K. Naruse;Y. Sakai;I. Nagashima;G. Jiang;M. Suzuki;T. Muto
我们以前开发了一种新型的无纺布生物人工肝生物反应器。我们还用肝细胞球体进行了实验，目的是提高这种NWF生物反应器的效率。在这项研究中，我们比较了使用肝细胞球体与单个肝细胞的NWF生物反应器的效率。采用Seglen法从全猪肝中分离肝细胞。1.0将× 1010个单个肝细胞固定在NWF生物反应器中。另外1.0 × 1010个肝细胞在4 L培养容器中悬浮培养24 h形成球状体，然后固定在生物反应器中。在NWF生物反应器中，发现肝细胞球状体在每个细胞的基础上在功能上上级单个肝细胞。然而，采用肝细胞球状体的NWF生物反应器表现出比采用单个肝细胞的NWF生物反应器更低的效率，因为在24小时悬浮培养期间肝细胞的总数已经减少。
We previously developed a new bioreactor of the bioartificial liver composed of non-woven fabric. We have also experimented with hepatocyte spheroids, with the aim of improving the efficiency of this NWF bioreactor. In this study, we compared the efficiencies of NWF bioreactors employing hepatocyte spheroids versus single hepatocytes. Hepatocytes were isolated from a whole pig liver by Seglen's method. 1.0 × 1010 single hepatocytes were immobilized in the NWF bioreactor. Another 1.0 × 1010 hepatocytes were allowed to form spheroids by 24 hr suspension culture in a 4-L culture vessel, before being immobilized in the bioreactor. Hepatocyte spheroids were found to be functionally superior, on a per-cell basis, to single hepatocytes in the NWF bioreactor. However, the NWF bioreactor employing hepatocyte spheroids exhibited lower efficiency than that employing single hepatocytes, because the total number of the hepatocytes had decreased during the 24 hr suspension culture.