QUANTIFICATION OF NORADRENALINE IONTOPHORESIS
QUANTIFICATION OF NORADRENALINE IONTOPHORESIS
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DOI：
10.1038/288181a0
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发表时间：
1980-01-01
期刊：
影响因子：
64.8
通讯作者：
KRUK, ZL
中科院分区：
文献类型：
--
作者：
ARMSTRONGJAMES, M;MILLAR, J;KRUK, ZL
当离子电渗法被用来研究假定的神经递质的作用时，遇到了几个问题。最重要的是，它通常是不切实际的，以估计在微电极的尖端通过一个给定强度的电流获得的药物浓度。通常的方法，很少使用，包括测量运输数，使用离子敏感的微电极和定量荧光显微镜。除了为乙酰胆碱开发的离子敏感微电极外，这些技术复杂且耗时，并且不能常规地应用于所使用的每一个电极。传统的多管微电极具有高阻抗记录桶，因此在记录单细胞活性时通常显示低信噪比，在离子电渗期间噪声增加。描述了一种技术，它在很大程度上克服了传统多管电极中的低尖峰信噪比的问题，并且与后者不同，它允许精确测定细胞环境中的去甲肾上腺素[去甲肾上腺素，NE]的浓度，这会影响其兴奋性。这些电极的记录和离子电渗性质先前已描述。使用这些电极，以精确地定量离子电渗NE通过适应极谱技术进行了描述。
Several problems are encountered when iontophoresis is used to study the effects of putative neurotransmitters. The most significant is that it is not usually practical to estimate the concentration of drug obtained at the tip of the microelectrode by a current of a given strength. The usual methods, rarely used, include measurement of transport numbers, the use of ion-sensitive microelecrodes and quantitative fluorescent microscopy. With the exception of the ion-sensitive microelectrodes developed for acetylcholine, these techniques are elaborate and time consuming and cannot be routinely applied to every electrode used. Conventional multibarrel microelectrodes have high-impedance recording barrels and thus often display low signal-to-noise ratios when recording single-cell activity, the noise being increased during iontophoresis. A technique is described which largely over comes the problem of low spike signal-to-noise ratio in conventional multibarrel electrodes, and which unlike the latter, allows precise determination of the concentration of noradrenaline [norepinephrine, NE] in the environment of the cell, which affects its excitability. The recording and iontophoretic properties of these electrodes were described previously. The use of these electrodes to quantify precisely iontophoresed NE by adapting polarographic techniques is described.