Antibiotics-induced depletion of mice microbiota induces changes in host serotonin biosynthesis and intestinal motility.
Antibiotics-induced depletion of mice microbiota induces changes in host serotonin biosynthesis and intestinal motility.
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抗生素引起的小鼠微生物群耗竭会引起宿主血清素生物合成和肠道蠕动的变化。
DOI：
10.1186/s12967-016-1105-4
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发表时间：
2017-01-13
影响因子：
7.4
通讯作者：
Li N
中科院分区：
文献类型：
--
作者：
Ge X;Ding C;Zhao W;Xu L;Tian H;Gong J;Zhu M;Li J;Li N
肠道菌群对胃肠运动的影响及其与肠道菌群之间的关系已成为人们关注的热点。然而，微生物群调节运动的机制尚未明确。因此，我们研究了抗生素对胃肠道运动、结肠血清素水平和胆汁酸代谢的微生物群消耗的影响。抗生素治疗4周后，分别测量和分析野生型和抗生素治疗小鼠的胃肠道和结肠运输、排便频率、水分含量和其他粪便参数。还分析了野生型和抗生素治疗小鼠的平滑肌收缩性、血清素水平和胆汁酸水平。抗生素处理后，小鼠肠道菌群丰富度和多样性显著降低，粪便排泄量减少（P < 0.01），水分含量增加（P < 0.01），颗粒长度增加（P < 0.01）。抗生素还导致小鼠胃肠和结肠运动延迟（P < 0.05），并抑制离体结肠近端纵肌的阶段性收缩（P < 0.01）。在抗生素治疗的小鼠中，血清素、色氨酸羟化酶1和次级胆汁酸水平降低。肠道菌群在调节肠道胆汁酸和血清素代谢中发挥重要作用，这可能是肠道菌群作为中间体与胃肠运动之间的关联。
The gastrointestinal motility is affected by gut microbiota and the relationship between them has become a hot topic. However, mechanisms of microbiota in regulating motility have not been well defined. We thus investigated the effect of microbiota depletion by antibiotics on gastrointestinal motility, colonic serotonin levels, and bile acids metabolism. After 4 weeks with antibiotics treatments, gastrointestinal and colon transit, defecation frequency, water content, and other fecal parameters were measured and analyzed in both wild-type and antibiotics-treated mice, respectively. Contractility of smooth muscle, serotonin levels, and bile acids levels in wild-type and antibiotics-treated mice were also analyzed. After antibiotics treatment, the richness and diversity of intestinal microbiota decreased significantly, and the fecal of mice had less output (P < 0.01), more water content (P < 0.01), and longer pellet length (P < 0.01). Antibiotics treatment in mice also resulted in delayed gastrointestinal and colonic motility (P < 0.05), and inhibition of phasic contractions of longitudinal muscle from isolated proximal colon (P < 0.01). In antibiotics-treated mice, serotonin, tryptophan hydroxylase 1, and secondary bile acids levels were decreased. Gut microbiota play an important role in the regulation of intestinal bile acids and serotonin metabolism, which could probably contribute to the association between gut microbiota and gastrointestinal motility as intermediates.