GAP-43, AFGF, CCK AND ALPHA-CGRP AND BETA-CGRP IN RAT SPINAL MOTONEURONS SUBJECTED TO AXOTOMY AND OR DORSAL-ROOT SEVERANCE
GAP-43, AFGF, CCK AND ALPHA-CGRP AND BETA-CGRP IN RAT SPINAL MOTONEURONS SUBJECTED TO AXOTOMY AND OR DORSAL-ROOT SEVERANCE
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DOI：
10.1111/j.1460-9568.1993.tb00918.x
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发表时间：
1993-10-01
影响因子：
3.4
通讯作者：
HOKFELT, T
中科院分区：
文献类型：
--
作者：
PIEHL, F;ARVIDSSON, U;HOKFELT, T
采用原位杂交法检测大鼠单侧周围神经切断、前根切断和背根切断后1、5、21天和20周腰段脊髓运动神经元生长相关蛋白43(GAP-43)、酸性成纤维细胞生长因子(AFGF)、降钙素基因相关肽(CGRP)、胆囊收缩素(CCK)和胆碱乙酰转移酶(ChAT)的mRNA水平。此外，用免疫组织化学方法研究了腹角内CGRP和aFGF样免疫反应。坐骨神经切断后1~21d，损伤侧运动神经元GAP-43和α-CGRP mRNAs表达增加，而损伤侧运动神经元aFGFmRNA表达水平较对照侧略高。另一方面，β-CGRP、CCK和ChAT的mRNA水平在轴突切断后的短期反应(1-21天)期间下降。前根切断术后，但不是外周神经根切断术后，病变近端的前根中aFGF样免疫反应完全消失。在外周神经切断后长期存活(20周)的动物中，所有研究物质的表达都恢复到正常水平。切断一侧背根不会引起损伤节段运动神经元GAP-43、aFGF、CGRP和CCK多肽或其mRNAs表达的短期或长期显著变化。这些结果表明，轴突切断后，运动神经元中的多肽/蛋白质表达存在差异。α-CGRP和GAP-43表达上调，CCK和β-CGRP表达下调，aFGF基本不受影响。
The mRNA levels for growth-associated protein 43 (GAP-43), acidic fibroblast growth factor (aFGF), alpha- and beta-calcitonin gene-related peptide (CGRP), cholecystokinin (CCK) and choline acetyltransferase (ChAT) in rat lumbar spinal motoneurons were studied by in situ hybridization 1, 5 and 21 days and 20 weeks following unilateral peripheral nerve sectioning, ventral rhizotomy or dorsal rhizotomy. Furthermore, CGRP- and aFGF-like immunoreactivities in the ventral horn were studied using immunohistochemistry. One to 21 days after axotomy, GAP-43 and alpha-CGRP mRNAs increased in lesioned motoneurons, while the aFGF mRNA levels were marginally higher in motoneurons on the lesion side as compared to the control side. Beta-CGRP, CCK and ChAT mRNA levels, on the other hand, decreased during the short-term response (1 - 21 days) to axotomy. After ventral rhizotomy, but not peripheral axotomy, there was complete disappearance of aFGF-like immunoreactivity in the ventral root proximal to the lesion. In animals subjected to long-term survival (20 weeks) after peripheral axotomy, the expression of all studied substances had returned to normal levels. Unilateral dorsal rhizotomy did not induce any substantial short- or long-term shifts in the cellular expression of the GAP-43, aFGF, CGRP and CCK peptides or their mRNAs in motoneurons of lesioned segments. These results indicate that peptides/proteins in motoneurons are expressed differentially after axotomy. Whereas alpha-CGRP and GAP-43 are up-regulated, CCK and beta-CGRP become down-regulated and aFGF is largely unaffected.