The role of FGF signaling in the establishment and maintenance of mesodermal gene expression in Xenopus
The role of FGF signaling in the establishment and maintenance of mesodermal gene expression in Xenopus
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DOI：
10.1002/dvdy.21517
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发表时间：
2008-05-01
影响因子：
2.5
通讯作者：
Harland, Richard M.
中科院分区：
文献类型：
--
作者：
Fletcher, Russell B.;Harland, Richard M.
FGF信号对于脊椎动物中胚层的形成是重要的，并且当其在爪蟾中受到干扰时，大多数躯干和尾部中胚层不能形成。在这里，我们进一步剖析了FGF的活动，通过解决其诱导和维护作用的胚胎图案。我们表明，FGF信号是必要的，除了其良好的特点，在维持xbra表达的作用，建立xbra表达。在非洲爪蟾中，FGF信号在组织者形成中的作用尚不清楚。我们发现，FGF信号是必不可少的近轴中胚层的初始规范，但不是激活几个泛中胚层和大多数组织者基因，然而，早期的FGF信号是必要的，以维持组织者基因表达到神经胚阶段。FGF信号传导的抑制防止特异性中胚层转录物的VegT活化。这些发现阐明了FGF信号如何有助于建立不同类型的中胚层。
FGF signaling is important for the formation of mesoderm in vertebrates, and when it is perturbed in Xenopus, most trunk and tail mesoderm fails to form. Here we have further dissected the activities of FGF in patterning the embryo by addressing its inductive and maintenance roles. We show that FGF signaling is necessary for the establishment of xbra expression in addition to its well-characterized role in maintaining xbra expression. The role of FGF signaling in organizer formation is not clear in Xenopus. We find that FGF signaling is essential for the initial specification of paraxial mesoderm but not for activation of several pan-mesodermal and most organizer genes; however, early FGF signaling is necessary for the maintenance of organizer gene expression into the neurula stage. Inhibition of FGF signaling prevents VegT activation of specific mesodermal transcripts. These findings illuminate how FGF signaling contributes to the establishment of distinct types of mesoderm.