Long-term preservation of germ cells and gonadal tissues at ambient temperatures.
Long-term preservation of germ cells and gonadal tissues at ambient temperatures.
复制标题
DOI：
10.1530/raf-22-0008
复制
发表时间：
2022-04-01
影响因子：
2.1
通讯作者：
Lee, Pei-Chih
中科院分区：
文献类型：
--
作者：
Comizzoli, Pierre;He, Xiaoming;Lee, Pei-Chih
综述了生殖细胞和性腺组织常温长期保存的不同方法和最新进展。现有文献综述。长期保存有活力的精子、卵子、胚胎和性腺组织对于人类生育治疗和动物种群（牲畜、生物医学模型和野生物种）的管理至关重要。在所有学科中，库存菌种的需求和数量都在快速增长，但目前在冷冻温度下的储存选择往往受到限制，并不总是可持续的。最近的研究表明，配子或性腺组织的结构和功能可以使用基于脱水和在超零度温度下储存的不同策略长期保存。然而，需要更多的研究在再水化和复苏的胚芽（包括适当的分子和细胞的评价）。虽然仍有大量研究需要优化每种样品类型和每种物种的干燥和再水化条件，但替代保存方法将改变生育力保存和生物库的范式。它将改变我们长期维护和管理珍贵生物材料的方式。活的精子、卵子、胚胎和生殖组织可以在冷冻温度下保存，用于人类生育治疗，并通过辅助生殖用于管理牲畜、实验室动物和野生物种的繁殖。这些细胞可以储存在细胞库中，对它们的需求正在快速增长。然而，目前在冷冻温度下的长期储存选择是昂贵的。最近的研究表明，这些细胞可以在冷冻温度以上干燥和储存很长一段时间，而不是使用低温。虽然仍然需要大量的研究来优化不同样本的干燥和再水化方式，但保存细胞的替代方法将使生育力保存和细胞库更容易。它还将改变我们长期保存和管理样本的方式。
To present an overview of different approaches and recent advances for long-term preservation of germ cells and gonadal tissues at ambient temperatures. Review of the existing literature. Preserving viable spermatozoa, eggs, embryos, and gonadal tissues for the long term is critical in human fertility treatment and for the management of animal populations (livestock, biomedical models, and wild species). The need and number of banked germplasms are growing very fast in all disciplines, but current storage options at freezing temperatures are often constraining and not always sustainable. Recent research indicates that structures and functions of gametes or gonadal tissues can be preserved for the long term using different strategies based on dehydration and storage at supra-zero temperatures. However, more studies are needed in rehydration and reanimation of germplasms (including proper molecular and cellular evaluations). While a lot of research is still warranted to optimize drying and rehydration conditions for each sample type and each species, alternative preservation methods will change the paradigm in fertility preservation and biobanking. It will transform the way we maintain and manage precious biomaterials for the long term. Living sperm cells, eggs, embryos, and reproductive tissues can be preserved at freezing temperatures for human fertility treatments and used to manage breeding in livestock, laboratory animals, and wild species through assisted reproduction. These cells can be stored in cell banks and demand for them is growing fast. However, current long-term storage options at freezing temperatures are expensive. Instead of using low temperatures, recent research indicates that these cells can be dried and stored above freezing temperatures for an extended amount of time. While a lot of research is still needed to optimize how different samples are dried and rehydrated, alternative methods of preserving cells will make fertility preservation and cell banking easier. It will also transform the way we keep and manage samples for the long term.