Granulocytic ehrlichiosis in the laboratory mouse
Granulocytic ehrlichiosis in the laboratory mouse
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DOI：
10.1086/514236
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发表时间：
1998-03-01
影响因子：
6.4
通讯作者：
Barthold, SW
中科院分区：
文献类型：
--
作者：
Hodzic, E;IJdo, JWI;Barthold, SW
接种从人粒细胞埃立克体病（HGE）患者分离的埃立克体的C3 H小鼠出现贫血和白细胞减少症，但到第24天，它们恢复到正常值。第5天和第10天，外周血和脾脏涂片中出现粒细胞桑椹胚，第17天桑椹胚减少。所有小鼠血液和脾脏的HL-60细胞培养物中均存在埃立克体。致病性，但不是感染性，减弱与小鼠传代，但可以复活的SCID小鼠传代。对血涂片法、HL-60细胞培养法、16 S重组DNA PCR扩增法、小鼠感染性试验等方法的敏感性进行了检测。所有方法均能在感染早期检测到血中HGE因子，但PCR和小鼠感染性试验在感染晚期最为敏感。异种诊断表明，小鼠在55天内保持持续感染。
C3H mice that were inoculated with ehrlichiae isolated from a patient with human granulocytic ehrlichiosis (HGE) developed anemia and leukopenia, but by day 24, they returned to normal values. Granulocytic morulae were present in peripheral blood and spleen smears on days 5 and 10, and there was a reduction in morulae on day 17. Ehrlichiae mere present in HL-60 cell cultures of blood and spleen from all mice at all intervals. Pathogenicity, but not infectivity, waned with mouse passage but could be resurrected by SCID mouse passage. Various methods were tested for their relative sensitivity in detecting infection: blood smears, HL-60 cell cultures, polymerase chain reaction (PCR) amplification of a 16S recombinant DNA target, and a mouse infectivity assay, All assays detected the HGE agent in blood during early infection, but PCR and the mouse infectivity assay were most sensitive during late infection. Xenodiagnosis demonstrated that mice remain persistently infected through 55 days.