Identification, accumulation, and functional prediction of novel tomato transcripts systemically upregulated after fire damage
Identification, accumulation, and functional prediction of novel tomato transcripts systemically upregulated after fire damage
复制标题
DOI：
10.1111/j.1399-3054.2005.00488.x
复制
发表时间：
2005-07-01
影响因子：
6.4
通讯作者：
Davies, E
中科院分区：
文献类型：
--
作者：
Coker, JS;Vian, A;Davies, E
尽管火灾对植物产生重大影响，但尚未在基因表达水平上仔细研究火灾损害的反应。在这里，我们对番茄（Lycopersicon esculentum cv. Heinz）的新转录本进行了分析，在远处的叶子被火焰伤害后，这些转录本在叶子中系统性上调。燃烧后 1 小时，从叶组织的消减 cDNA 文库中分离出 9 个 cDNA 片段。使用数据挖掘和聚合酶链式反应 (PCR)，预测、扩增全长开放阅读框，然后进行测序。燃烧后使用叶 RNA 进行的实时 (RT)-PCR 证实分别在 30 分钟和 60 分钟内系统积累了 4 个和 7 个转录物，然后在 3 小时内恢复到基础水平。在同一时间过程中，蛋白酶抑制剂 I 的水平在 6 小时内逐渐增加了 30 倍以上。表达分析还表明，其中八个转录物存在于未受伤的叶、茎和根组织中。预测的蛋白质包括酰基载体、腺苷酸硫酸还原酶、PS II 释氧复合蛋白 3、阴离子：钠同向转运蛋白、叶绿体特异性核糖体蛋白、组氨酸三联体家族蛋白和未知的伤口/应激相关蛋白。其中几种蛋白质的同源物与其他类型的伤口和应激反应有关。看来，在被火损坏后一小时内，除了蛋白酶抑制剂等经典防御基因外，植物还会系统性地上调多种与基本细胞代谢和维持有关的基因。
Despite the major impacts of fire on plants, responses to fire damage have not been closely studied on the level of gene expression. Here, we present analyses of novel transcripts from tomato (Lycopersicon esculentum cv. Heinz), which are systemically upregulated in leaves after a distant leaf is wounded by flame. Nine cDNA fragments were isolated from a subtractive cDNA library of leaf tissue 1 h after flaming. Using data mining and polymerase chain reaction (PCR), full-length open-reading frames were predicted, amplified, and then sequenced. Real-time (RT)-PCR using leaf RNA after flaming confirmed the systemic accumulation of 4 and 7 transcripts within 30 and 60 min, respectively, before returning to basal levels within 3 h. During this same time course, proteinase inhibitor I levels gradually increased over 30-fold in 6 h. Expression analyses also showed that eight of the transcripts are present in unwounded leaf, stem, and root tissues. The predicted proteins include an acyl carrier, adenylyl sulfate reductase, PS II oxygen-evolving complex protein 3, anion : sodium symporter, chloroplast-specific ribosomal protein, a histidine triad family protein, and an unknown wound/stress-related protein. Homologs of several of these proteins have been associated with other types of wound and stress responses. It appears that, within an hour after being damaged by fire, plants systemically upregulate a variety of genes involved with basic cell metabolism and upkeep, in addition to classic defense genes such as proteinase inhibitors.