Bronchoalveolar lavage and histologic characterization of late asthmatic response in guinea pigs.
Bronchoalveolar lavage and histologic characterization of late asthmatic response in guinea pigs.
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豚鼠晚期哮喘反应的支气管肺泡灌洗和组织学特征。
DOI：
10.1164/ajrccm/136.4.922
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发表时间：
1987
期刊：
影响因子：
--
通讯作者：
T. Takishima
中科院分区：
文献类型：
--
作者：
H. Iijima;M. Ishii;K. Yamauchi;C. Chao;K. Kimura;S. Shimura;Y. Shindoh;H. Inoue;S. Mue;T. Takishima
为了阐明在哮喘受试者中观察到的迟发性哮喘反应（LAR）的机制，我们使用豚鼠建立了LAR动物模型。用猪蛔虫提取物和氢氧化铝的混合物免疫50只豚鼠，然后在没有麻醉的情况下吸入猪蛔虫提取物进行攻击。50只豚鼠中有20只表现出双重哮喘反应，其中LAR发生在立即哮喘反应（IAR）后3至6 h。通过液氮快速冷冻或支气管肺泡灌洗（BAL）对20只LAR豚鼠中的14只进行了组织学研究，并与18只IAR豚鼠中的10只、10只对照豚鼠和10只未免疫但激发的豚鼠进行了比较。LAR豚鼠BAL液中中性粒细胞的百分比和绝对数量均显著高于对照豚鼠（p <0.02）和未免疫但激发的豚鼠（p <0.02）。然而，LAR豚鼠与仅IAR的豚鼠无显著差异。另一方面，组织学检查表明，嗜酸性粒细胞浸润的豚鼠与LAR的气道壁内比豚鼠与仅IAR，并表明有没有显着差异，中性粒细胞浸润的豚鼠与LAR和动物之间的气道壁内仅IAR。在LAR中还观察到气道（支气管、细支气管）平滑肌收缩、粘膜下水肿和气道腔中的粘液。（250字处删节）
To elucidate the mechanisms of late asthmatic response (LAR) observed in asthmatic subjects, we have developed an animal model of LAR using guinea pigs. Fifty guinea pigs were immunized with a mixture of Ascaris suum extract and aluminum hydroxide and then challenged with an inhalation of Ascaris suum extract without anesthesia. Twenty of the 50 guinea pigs showed a dual asthmatic response in which the LAR occurred 3 to 6 h after immediate asthmatic response (IAR). Histologic studies by rapid freezing with liquid nitrogen or bronchoalveolar lavage (BAL) were performed in 14 of these 20 guinea pigs with LAR and compared with those in 10 of 18 guinea pigs with only IAR, 10 control guinea pigs, and 10 nonimmunized but challenged guinea pigs. Both the percentage and the absolute number of neutrophils in the BAL fluid of the guinea pigs with LAR were significantly greater than those of the control guinea pigs (p less than 0.02) and than those of the nonimmunized but challenged guinea pigs (p less than 0.02). However, that of guinea pigs with LAR was not significantly different from that of guinea pigs with only IAR. On the other hand, histologic examination showed that eosinophil infiltration within the airway walls of the guinea pigs with LAR was more prominent than that of the guinea pigs with only IAR, and showed that there was no significant difference in neutrophil infiltration within the airway walls between the guinea pigs with LAR and the animals with only IAR. Contraction of airway (bronchus, bronchiole) smooth muscle, submucosal edema, and mucus in airway lumen were also observed in LAR.(ABSTRACT TRUNCATED AT 250 WORDS)