Multifunctional activity of recombinant p14 on lymphoid cell cultures.
Multifunctional activity of recombinant p14 on lymphoid cell cultures.
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重组 p14 对淋巴细胞培养物的多功能活性。
DOI：
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发表时间：
2000
影响因子：
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通讯作者：
A. Savarino
中科院分区：
文献类型：
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作者：
A. Pugliese;D. Torre;C. Cantamessa;A. Saini;M. Pes;A. Savarino
研究了免疫缺陷病毒1型(HIV-1)Tat基因编码的重组p14蛋白对T淋巴细胞培养的影响。特别是，我们检测了经p14处理的HIV-1感染或未感染的MT-4和H9细胞对p14的吸附、细胞复制率、纤维连接蛋白(FN)及其受体(FNR)和细胞表面CD4抗原的表达。此外，我们还评估了未感染和慢性感染的H9细胞在p14存在下的凋亡细胞比例以及p14对健康受试者PBMC中干扰素(干扰素)产生的调节作用。这些结果表明，p14对HIV-1感染和未感染的细胞具有多功能活性。特别是，该蛋白以一种特定的方式与细胞表面相互作用，特别是与感染细胞的表面相互作用，并增强FN和FNR的表达，但不增强CD4淋巴细胞抗原的表达。此外，p14可促进细胞复制和干扰素的产生，并对细胞凋亡起到轻微的调节作用。我们还提出了一个模型来解释外源性p14在HIV-1感染中的可能作用。
Some effects of recombinant p14, a protein encoded by the tat gene of immunodeficiency virus type 1 (HIV-1), were investigated on T lymphocytic cell cultures. In particular, we detected p14 adsorption to cells, the rate of cell replication, the expression of fibronectin (FN) and its receptor (FNR) and of cell surface CD4 antigen in HIV-1-infected or uninfected MT-4 and H9 cells, treated with p14. Moreover, we evaluated the proportion of apoptotic cells in uninfected and chronically infected H9 cells in the presence of p14 and the modulation of interferon (IFN) production induced by p14 in PBMC of healthy subjects. The results obtained demonstrate that p14 exerts multifunctional activities on HIV-1 infected and uninfected cells. In particular, this protein interacts in a specific manner with cell surface, especially with that of infected cells, and enhances the expression of FN and FNR but not that of the CD4 lymphocyte antigen. Moreover, p14 increases cell replication, IFN production and can exert a slight modulation of apoptosis. We also propose a model to explain a possible role in HIV-1 infection of the effects of exogenous p14.