Structured spike series specify gene expression patterns for olfactory circuit formation
Structured spike series specify gene expression patterns for olfactory circuit formation
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DOI：
10.1126/science.aaw5030
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发表时间：
2019-07-05
期刊：
影响因子：
56.9
通讯作者：
Takeuchi, Haruki
中科院分区：
文献类型：
--
作者：
Nakashima, Ai;Ihara, Naoki;Takeuchi, Haruki
神经回路通过遗传程序和活动依赖过程的相互作用而出现。在小鼠嗅觉图谱的发育过程中，轴突以嗅觉受体（OR）依赖的方式分离成不同的肾小球。ORs生成轴突分类分子的组合代码，其表达受神经活动调节。然而，尚不清楚神经活动如何诱导轴突分选分子的or特异性表达模式。我们发现自发性神经元峰值的时间模式没有空间组织，但与OR类型相关。受体替代实验表明，ORs决定自发活动模式。此外，光遗传分化的神经元活动模式诱导相应轴突分选分子的特异性表达并调节轴突分离。因此，在嗅觉图谱形成过程中，依赖于or的自发活动的时间模式在产生轴突分选分子的组合密码中发挥了指导作用。
Neural circuits emerge through the interplay of genetic programming and activity-dependent processes. During the development of the mouse olfactory map, axons segregate into distinct glomeruli in an olfactory receptor (OR)-dependent manner. ORs generate a combinatorial code of axon-sorting molecules whose expression is regulated by neural activity. However, it remains unclear how neural activity induces OR-specific expression patterns of axon-sorting molecules. We found that the temporal patterns of spontaneous neuronal spikes were not spatially organized but were correlated with the OR types. Receptor substitution experiments demonstrated that ORs determine spontaneous activity patterns. Moreover, optogenetically differentiated patterns of neuronal activity induced specific expression of the corresponding axon-sorting molecules and regulated axonal segregation. Thus, OR-dependent temporal patterns of spontaneous activity play instructive roles in generating the combinatorial code of axon-sorting molecules during olfactory map formation.