Aequorivita lutea sp. nov., a novel bacterium isolated from the estuarine sediment of the Pearl River in China, and transfer of Vitellibacter todarodis and Vitellibacter aquimaris to the genus Aequorivita as Aequorivita todarodis comb. nov. and Aequorivit
Aequorivita lutea sp. nov., a novel bacterium isolated from the estuarine sediment of the Pearl River in China, and transfer of Vitellibacter todarodis and Vitellibacter aquimaris to the genus Aequorivita as Aequorivita todarodis comb. nov. and Aequorivit
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DOI：
10.1099/ijsem.0.004139
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发表时间：
2020-04
影响因子：
2.8
通讯作者：
Shuangfei Zhang;Haixin Zhou;Chongran Sun;Zhong Hu;Hui Wang
中科院分区：
文献类型：
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作者：
Shuangfei Zhang;Haixin Zhou;Chongran Sun;Zhong Hu;Hui Wang
从中国珠江河口沉积物中分离出一种革兰氏阴性、好氧、杆状、非运动性滑行细菌，并将其命名为菌株q18T。培养48小时后，在海洋琼脂上菌落呈圆形、光滑、黄色。最佳生长的盐度、温度和 pH 值分别为 5 % (NaCl)、30 °C 和 7。菌株q18T的16S rRNA基因序列与模式菌株Aequorivita einoideorum CC-CZW007T的相似性最高，达97.3%。基于16S rRNA基因的系统发育分析表明，菌株q18T属于拟杆菌门黄杆菌科的Aequorivita属，并且与该属中所有已知物种不同。甲基萘醌 (MK-6) 是在 q18T 菌株中检测到的主要呼吸醌。主要脂肪酸为 iso-C15 : 0 和 iso-C17 : 0 3-OH。菌株q18T的极性脂质主要包括磷脂酰乙醇胺、两种未鉴定的氨基脂质、两种未鉴定的磷脂和一种未鉴定的极性脂质。基因组的 G+C 含量约为 42.8 mol%。菌株 q18T 的基因组草案大小为 3.3 Mbp。菌株 q18T 和参考 Aequorivita 菌株之间的平均核苷酸同一性值约为 79.0%。基于多相分析，菌株 q18T 被确认为 Aequorivita 属的一个新种，其名称为 Aequorivita utea sp。十一月，提出。模式菌株为q18T（=CICC 24821T=KCTC 72764 T）。此外，根据系统发育、化学分类学和表型分析的结果，先前分类为Vitellibacter属的两个物种，Vitellibacter todarodis Kim等人。 2018 和 Vitellibacter aquimaris Thevarajoo 等人。 2016年，被转移到Aequorivita属，命名为Aequorivita todarodis 梳子。十一月和 Aequorivita aquimaris 梳子。十一月分别。
A Gram-negative, aerobic, rod-shaped, non-motile by gliding bacterium was isolated from the estuarine sediment of the Pearl River in PR China and designated as strain q18T. Colonies were circular, smooth and yellow on marine agar after 48 h cultivation. Salinity, temperature and pH for optimal growth were 5 % (NaCl), 30 °C and 7, respectively. The 16S rRNA gene sequence of the strain q18T showed the highest similarity of 97.3 % to the type strain of Aequorivita echinoideorum CC-CZW007T. 16S rRNA gene-based phylogenetic analysis indicated that strain q18T grouped into the genus Aequorivita in the family Flavobacteriaceae of the phylum Bacteroidetes, and was distinct from all known species in the genus. Menaquinone (MK-6) was the main respiratory quinone detected in strain q18T. The major fatty acids were iso-C15 : 0 and iso-C17 : 0 3-OH. The polar lipids of strain q18T mainly comprised phosphatidylethanolamine, two unidentified aminolipids, two unidentified phospholipids and one unidentified polar lipid. The G+C content of the genome was ~42.8 mol%. The draft genome size of strain q18T was 3.3 Mbp. The average nucleotide identity values were around 79.0 % between strain q18T and reference Aequorivita strains. Based on the polyphasic analysis, strain q18T was confirmed to represent a novel species of the genus Aequorivita, for which the name Aequorivita lutea sp. nov., is proposed. The type strain is q18T (=CICC 24821T=KCTC 72764 T). Further, based on the results of phylogenetic, chemotaxonomic and phenotypic analyses, two species previously classified into the genus Vitellibacter, Vitellibacter todarodis Kim et al. 2018 and Vitellibacter aquimaris Thevarajoo et al. 2016, are transferred to the genus Aequorivita as Aequorivita todarodis comb. nov. and Aequorivita aquimaris comb. nov. respectively.