Strategies and technologies for exploring long noncoding RNAs in heart failure
Strategies and technologies for exploring long noncoding RNAs in heart failure
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探索心力衰竭中长非编码 RNA 的策略和技术
DOI：
10.1016/j.biopha.2020.110572
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发表时间：
2020
影响因子：
7.5
通讯作者：
Weidong Zhang
中科院分区：
文献类型：
--
作者：
Zhen Zhang;Jingjing Wan;Xia Liu;Weidong Zhang
长的非编码RNA(LncRNA)曾被认为是基因组转录的噪音，没有生物功能。然而，越来越多的证据表明，lncRNA在发育阶段或疾病状态下动态表达，在基因表达和翻译过程中发挥调节作用。近年来，LncRNA被认为是控制心脏的功能调控网络的核心节点，并参与心肌肥大、纤维化、血管生成等多个心力衰竭过程，有望成为治疗疾病的靶点。事实上，正是技术的发展提高了我们对lncRNA的理解，拓宽了我们对心力衰竭的看法。从转录噪声到恒星分子，如果没有多学科技术的结合，尤其是高通量方法的出现，lncRNAs的进展是不可能实现的。因此，我们在此综述了可用于探索lncRNAs的策略和技术，并试图对lncRNAs在心力衰竭的临床诊断和治疗中的前景有所了解。
Long non-coding RNA (lncRNA) was once considered to be the "noise" of genome transcription without biological function. However, increasing evidence shows that lncRNA is dynamically expressed in developmental stage or disease status, playing a regulatory role in the process of gene expression and translation. In recent years, lncRNA is considered to be a core node of functional regulatory networks that controls cardiac and also involves in multiple process of heart failure such as myocardial hypertrophy, fibrosis, angiogenesis, etc., which would be a therapeutic target for diseases. In fact, it is the development of technology that has improved our understanding of lncRNAs and broadened our perspective on heart failure. From transcriptional "noise" to star molecule, progress of lncRNAs can't be achieved without the combination of multidisciplinary technologies, especially the emergence of high-throughput approach. Thus, here, we review the strategies and technologies available for the exploration lncRNAs and try to yield insights into the prospect of lncRNAs in clinical diagnosis and treatment in heart failure.