Regulated expression of the tyrosine hydroxylase gene by epidermal growth factor.
Regulated expression of the tyrosine hydroxylase gene by epidermal growth factor.
复制标题
表皮生长因子调节酪氨酸羟化酶基因的表达。
DOI：
10.1128/mcb.7.9.3332-3336.1987
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发表时间：
1987
影响因子：
5.3
通讯作者：
Chikaraishi,DM
中科院分区：
文献类型：
--
作者：
Lewis,EJ;Chikaraishi,DM
此外，表皮生长因子（EGF）的大鼠PCG 2嗜铬细胞瘤细胞系的培养物增加了酪氨酸羟化酶（TH）的RNA编码的水平。将含有TH基因5′侧翼序列的DNA片段与异源基因融合，转染大鼠垂体前叶细胞系GH 4。从+27到-272的TH基因序列包含足够的信息，用于EGF诱导TH。这TH DNA中的两个区域与大鼠催乳素基因的EGF调节元件广泛同源。
The addition of epidermal growth factor (EGF) to cultures of the rat PCG2 pheochromocytoma cell line increased the level of RNA coding for tyrosine hydroxylase (TH). A region of DNA containing 5′-flanking sequences of the TH gene was fused to a heterologous gene and transfected into a rat anterior pituitary cell line, GH4. The TH gene sequences from +27 to -272 contained information sufficient for the induction of TH by EGF. Two regions within this TH DNA were extensively homologous to the EGF regulatory element of the rat prolactin gene.