Sperm mRNA screening by reverse transcription-recombinase polymerase amplification (RT-RPA) assay: Decision-making for differential extraction
Sperm mRNA screening by reverse transcription-recombinase polymerase amplification (RT-RPA) assay: Decision-making for differential extraction
复制标题
通过逆转录重组酶聚合酶扩增 (RT-RPA) 检测进行精子 mRNA 筛选：差异提取决策
DOI：
10.1016/j.ab.2023.115121
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发表时间：
2023
影响因子：
2.9
通讯作者：
Kitajima Isao
中科院分区：
文献类型：
--
作者：
Kubo Seiji;Niimi Hideki;Kitajima Isao
精子细胞的存在是性侵犯样本差异提取的一个指标。一般来说，精子细胞通过显微镜分析鉴定;然而，这种传统方法需要时间和精力，即使是受过训练的人员。在这里，我们提出了一种逆转录-重组酶聚合酶扩增（RT-RPA）检测针对精子mRNA标记物（PRM 1）。RT-RPA检测PRM 1仅需40 min，灵敏度为0.1 μL精液。我们的研究结果表明，RT-RPA检测可能是一种快速，简单，特异性的策略，用于筛选性侵犯样本中的精子细胞。
The presence of sperm cells is an indicator for differential extraction on sexual assault samples. In general, sperm cells are identified by microscopic analysis; however, this conventional method takes time and effort, even for trained personnel. Here, we present a reverse transcription-recombinase polymerase amplification (RT-RPA) assay targeting sperm mRNA marker (PRM1). The RT-RPA assay requires only 40 min forPRM1detection and demonstrates a sensitivity of 0.1 μL of semen. Our results indicate that the RT-RPA assay may be a rapid, simple, and specific strategy for screening sperm cells in sexual assault samples.