Mitf contributes to melanosome distribution and melanophore dendricity
Mitf contributes to melanosome distribution and melanophore dendricity
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DOI：
10.1111/j.1755-148x.2007.00420.x
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发表时间：
2008-02-01
影响因子：
4.3
通讯作者：
Yamamoto, Hiroaki
中科院分区：
文献类型：
--
作者：
Kawasaki, Akiha;Kumasaka, Mayuko;Yamamoto, Hiroaki
MITF是碱性/螺旋-环-螺旋/亮氨酸拉链家族的转录因子，对黑素细胞和视网膜色素上皮的发育是不可或缺的。我们以前以非洲爪哇为模型系统的工作表明，MITF调节黑素小体在体内的扩散，尽管这是通过黑素小体运输还是通过黑素载体树枝状分布并不明显。为了更好地了解MITF的作用，我们现在鉴定了野生型MITF注射或显性阴性MITF注射的胚胎的神经管培养，并将它们与对照组进行比较。在体外，较低水平的MITF活性诱导较少的树突状黑素载体聚集的黑素小体，而高表达MITF的黑素载体具有广泛的树突状形态，分散的黑素小体。此外，免疫荧光分析显示，显性负性MITF的表达导致Rab27a表达下降。这些结果表明，MITF参与了黑素小体运输的调节和黑素载体中树突的水平。
Mitf is a transcription factor of the basic/helix-loop-helix/leucine-zipper family which is indispensable for development of melanocytes and the retinal pigment epithelium. Our previous work using Xenopus laevis as a model system suggested that Mitf regulates melanosome dispersal in vivo though whether this was via melanosome transport or melanophore dendricity was not obvious. To better understand the role of Mitf, we have now characterized neural tube cultures from wild-type Mitf-injected or a dominant-negative Mitf-injected embryos and compared them with controls. In vitro, lower levels of Mitf activity induced less dendritic melanophores with aggregated melanosomes, whereas melanophores overexpressing Mitf had an extensive dendritic morphology with dispersed melanosomes. Moreover, immunorfluoresence assays reveal that expression of a dominant-negative Mitf leads to decreased Rab27a expression. These results suggest that Mitf is involved in the regulation of melanosome transport and the level of dendricity in melanophores.