THE PREDICTED AMINO-ACID-SEQUENCE OF HUMAN THIOREDOXIN IS IDENTICAL TO THAT OF THE AUTOCRINE GROWTH-FACTOR HUMAN ADULT T-CELL DERIVED FACTOR (ADF) - THIOREDOXIN MESSENGER-RNA IS ELEVATED IN SOME HUMAN TUMORS
THE PREDICTED AMINO-ACID-SEQUENCE OF HUMAN THIOREDOXIN IS IDENTICAL TO THAT OF THE AUTOCRINE GROWTH-FACTOR HUMAN ADULT T-CELL DERIVED FACTOR (ADF) - THIOREDOXIN MESSENGER-RNA IS ELEVATED IN SOME HUMAN TUMORS
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DOI：
10.1016/0167-4781(94)90180-5
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发表时间：
1994-08-02
期刊：
影响因子：
--
通讯作者：
POWIS, G
中科院分区：
文献类型：
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作者：
GASDASKA, PY;OBLONG, JE;POWIS, G
已发现通过PCR克隆从人结肠癌细胞、人淋巴母细胞样细胞和人肝脏获得的硫氧还蛋白的cDNA序列与报道的自分泌生长因子、人成人T细胞白血病衍生因子（ADF）的cDNA序列相同。重组人硫氧还蛋白被二硫苏糖醇还原95%，是人硫氧还蛋白还原酶还原的底物。与配对的正常人肺组织中的硫氧还蛋白mRNA相比，手术时不吸烟的受试者的人非小细胞原发性肺肿瘤显示硫氧还蛋白mRNA水平显著增加。吸烟者的肺肿瘤硫氧还蛋白mRNA没有显著增加。研究结果表明，人类硫氧还蛋白和ADF是相同的物种，并表明某些人类癌症可能会增加硫氧还蛋白（ADF）的产生。
The cDNA sequences of thioredoxin obtained by PCR cloning from human colon cancer cells, human lymphoblastoid cells, and human liver have been found to be identical with the cDNA sequence reported for the autocrine growth factor, human adult T-cell leukemia derived factor (ADF). Recombinant human thioredoxin was 95% reduced by dithiothreitol and was a substrate for reduction by human thioredoxin reductase. Human non-small cell primary lung tumors from subjects who were not cigarette smokers at the time of surgery showed significantly increased levels of thioredoxin mRNA compared to thioredoxin mRNA in paired normal human lung tissue. Subjects who were smokers did not show a significant increase in lung tumor thioredoxin mRNA. The results of the study show that human thioredoxin and ADF are identical species and suggest that there may be increased production of thioredoxin (ADF) by some human cancers.