Effect of the surface characteristics of Methanosarcina barkeri on immobilization to support materials
Effect of the surface characteristics of Methanosarcina barkeri on immobilization to support materials
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DOI：
10.1163/156855207782146599
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发表时间：
2007
影响因子：
5.2
通讯作者：
T. Nomura;Akinori Yoshihara;Takanori Nagao;H. Tokumoto;Y. Konishi
中科院分区：
文献类型：
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作者：
T. Nomura;Akinori Yoshihara;Takanori Nagao;H. Tokumoto;Y. Konishi
摘要为了实现高效厌氧处理，需要在发酵罐中培养高浓度的产甲烷菌。在这项研究中，我们实验研究的效果的表面特性的乙酸利用甲烷八叠球菌Barkeri（JCM 10043）固定到支持材料。为此，我们测量了M的静电和疏水性质。巴克里。测定了M. barkeri随着细胞悬浮液离子强度的增加而降低，并通过Ohshima方程使用两个参数进行拟合：在λ区域的空间电荷密度（ZeN = -1.15 × 10 6 C/m3）和柔软度参数（1/λ = 3.35 × 10 −9 m）。M.巴克氏体对正十六烷具有亲和力，粘附率大于60%。M.巴氏杆菌表现出相对于大肠杆菌的疏水性。我们还进行了微生物粘附测试以支持材料。M. Barkeri对阴离子交换树脂的粘附性比对疏水性树脂的粘附性好。微生物细胞均匀地粘附在阴离子交换树脂上，而凝固的细胞不均匀地粘附在疏水性树脂上。M. Barkeri显示出对阳离子交换树脂的粘附性差。阴离子交换树脂对M.发酵罐内的巴克里。
Abstract To realize a highly efficient anaerobic treatment, it is necessary to immobilize high concentrations of methanogens within a fermenter. In this study, we experimentally examine the effect of the surface characteristics of the acetate-utilizing methanogen Methanosarcina barkeri (JCM 10043) on immobilization to support materials. To this aim, we measured the electrostatic and hydrophobic properties of M. barkeri . The electrophoretic mobility of M. barkeri decreased with increasing ionic strength of the cell suspension and was fitted by the Ohshima equation using two parameters: spatial charge density in the polyelectrolyte region ( ZeN = –1.15 × 10 6 C/m 3 ) and the softness parameter (1/λ = 3.35 × 10 −9 m). M. barkeri showed an affinity to n -hexadecane, with adhesion of more than 60%. M. barkeri showed hydrophobicity relative to Escherichia coli . We also carried out a microbial adhesion test to support materials. M. barkeri showed better adhesion to the anion-exchange resin than to the hydrophobic resin. The microbial cells adhered uniformly to the anion-exchange resin, while coagulated cells adhered non-uniformly to the hydrophobic resin. M. barkeri showed poor adhesion to the cation-exchange resin. The anion-exchange resin is most effective in immobilizing M. barkeri within the fermenter.