Cell patterning using magnetite nanoparticles and magnetic force
Cell patterning using magnetite nanoparticles and magnetic force
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DOI：
10.1002/bit.21322
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发表时间：
2007-08-01
影响因子：
3.8
通讯作者：
Honda, Hiroyuki
中科院分区：
文献类型：
--
作者：
Ino, Kosuke;Ito, Akira;Honda, Hiroyuki
通过精确地使细胞图案化来制造功能性组织架构的技术对于组织工程是高度期望的。虽然已经开发了几种细胞图案化方法，例如微接触印刷和光刻，但这些方法需要专用表面用作基底，其制造是耗时的。在本研究中，我们展示了一种简单快速的细胞图案化技术，使用磁铁矿纳米颗粒和磁力，使我们能够在任意表面上分配细胞。本研究利用我们前期研究开发的磁性阳离子脂质体（MCLs）对靶细胞进行磁性标记。当将置于磁体上的钢板置于细胞培养表面下时，磁性标记的细胞排列在钢板所处的表面上。通过调整接种细胞的数量实现了单个细胞的图案化线，并成功地制造了复杂的细胞图案（弯曲、平行或交叉图案）。由于使用磁力的细胞图案化可能不限制培养表面的性质，因此将人脐静脉内皮细胞（HUVEC）在基质胶上图案化，从而形成图案化的毛细血管。这些结果表明，新的细胞图案化的方法，它使用MCL，是一种有前途的方法，组织工程和研究细胞-细胞之间的相互作用在体外。
Technologies for fabricating functional tissue architectures by patterning cells precisely are highly desirable for tissue engineering. Although several cell patterning methods such as microcontact printing and lithography have been developed, these methods require specialized surfaces to be used as substrates, the fabrication of which is time consuming. In the present study, we demonstrated a simple and rapid cell patterning technique, using magnetite nanoparticles and magnetic force, which enables us to allocate cells on arbitrary surfaces. Magnetite cationic liposomes (MCLs) developed in our previous study were used to magnetically label the target cells. When steel plates placed on a magnet were positioned under a cell culture surface, the magnetically labeled cells lined on the surface where the steel plate was positioned. Patterned lines of single cells were achieved by adjusting the number of cells seeded, and complex cell patterns (curved, parallel, or crossing patterns) were successfully fabricated. Since cell patterning using magnetic force may not limit the property of culture surfaces, human umbilical vein endothelial cells (HUVECs) were patterned on Matrigel, thereby forming patterned capillaries. These results suggest that the novel cell patterning methodology, which uses MCLs, is a promising approach for tissue engineering and studying cell-cell interactions in vitro.