Quantification of choline and ethanolamine phospholipids in rabbit myocardium.
Quantification of choline and ethanolamine phospholipids in rabbit myocardium.
复制标题
兔心肌中胆碱和乙醇胺磷脂的定量。
DOI：
10.1016/0003-2697(85)90084-3
复制
发表时间：
1985
影响因子：
2.9
通讯作者：
Sobel,BE
中科院分区：
文献类型：
--
作者：
Creer,MH;Pastor,C;Corr,PB;Gross,RW;Sobel,BE
为了便于评估心肌磷脂代谢对缺血引起的电生理异常的影响，开发了一种用于定量胆碱和乙醇胺磷脂的方法，该方法适用于对少量心肌进行准确和可重复的分析。该程序结合了组织匀浆后的氯仿和甲醇提取磷脂，随后通过连续薄层色谱法和高效液相色谱法进行分离。磷纯化的脂质类进行了测定与回收率校正的基础上14 C-标记的内标物。
To facilitate evaluation of the influence of myocardial phospholipid metabolities on the development of electrophysiologic abnormalities induced by ischemia, a method for the quantification of choline and ethanolamine phospholipids suitable for accurate and reproducible analysis of small amounts of myocardium was developed. The procedure combines chloroform and methanol extraction of phospholipids after tissue homogenization with subsequent separation by sequential thin-layer and high-performance liquid chromatography. Phosphorus in purified lipid classes was determined with the correction for recovery based on14C-labeled internal standards.