RecQ helicase enhances homologous recombination in plants
RecQ helicase enhances homologous recombination in plants
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DOI：
10.1016/j.febslet.2004.08.020
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发表时间：
2004-09
期刊：
影响因子：
3.5
通讯作者：
Hongqing Li;Mei-Ru Li
中科院分区：
文献类型：
--
作者：
Hongqing Li;Mei-Ru Li
RecQ解旋酶是大肠杆菌RecF途径中启动同源重组的关键组分。在这里，我们证明了RecQ基因在水稻胚性细胞中的瞬时表达使同源重组效率提高了4倍之多。进一步的实验表明，这种效果受到RecQ剂量的影响。RecQ在水稻中的稳定表达使来自不同转基因系的叶组织中的同源重组事件显著增加20至40倍。这是首次证明RecQ基因的过表达可以刺激植物中的同源重组。
RecQ helicase is a key component in the RecF pathway of Escherichia coli for initiation of homologous recombination. Here, we demonstrate that transient expression of RecQ gene in rice embryogenic cell increases the homologous recombination efficiency as much as 4-fold. Further experiments reveal that this effect is influenced by the RecQ dosage. Stable expression of RecQ in rice dramatically increases the homologous recombination events 20- to 40-fold in leaf tissue from different transgenic lines. This is the first evidence indicating that overexpression of RecQ gene can stimulate homologous recombination in plants.