Carcinogens induce intrachromosomal recombination in yeast.
Carcinogens induce intrachromosomal recombination in yeast.
复制标题
致癌物诱导酵母染色体内重组。
DOI：
10.1093/carcin/10.8.1445
复制
发表时间：
1989
期刊：
影响因子：
4.7
通讯作者：
Hastings,PJ
中科院分区：
文献类型：
--
作者：
Schiestl,RH;Gietz,RD;Mehta,RD;Hastings,PJ
为了确定环境致癌物，需要廉价和可靠的短期测试，可以用来预测任何给定物质的致癌潜力，具有高准确性。艾姆斯试验基于鼠伤寒沙门氏菌的突变诱导，是最广泛使用的短期试验，但存在某些人类或动物致癌物，这些致癌物作为诱变剂，在艾姆斯试验或其他短期试验中持续检测不到。需要一种短期试验来检测那些被艾姆斯试验遗漏的致癌物。在许多情况下，致癌作用与基因组重排有关。由于这种关联，已经构建了一个筛选导致基因组重排的染色体内重组的系统，该系统可能用作酿酒酵母中的短期测试。对该重组系统的评价表明，它容易被各种致突变性和非致突变性致癌物诱导，包括通过艾姆斯试验或各种其他短期试验检测不到的致癌物，如黄樟素、氨基甲酸乙酯、乙醇、金胺、二氯甲烷、四氯化碳、硫酸镉、苯胺、二甲基肼、氨基三唑、乙酰胺、硫脲和DDE。本报告显示了这些药物以及其他药物的数据、不同浓度药物的响应曲线以及DEL系统的方案。
To identify environmental carcinogens there is a need for inexpensive and reliable short-term tests that can be used to predict the carcinogenic potential of any given substance with high accuracy. The Ames assay, which is based on the induction of mutations inSalmonella typhimurium, is the most extensively used short-term test but certain human or animal carcinogens exist that are persistently undetectable as mutagens with the Ames assay or with other short-term tests. There is a need for a short-term test to detect those carcinogens that are missed by the Ames assay. Carcinogenesis is in many cases associated with genome rearrangement. Because of this association a system screening for intra-chromosomal recombination that results in genome rearrangement has been constructed for potential use as a short-term test in the yeastSaccharomyces cerevisiae. Evaluation of this recombination system shows that it is readily inducible by a variety of mutagenic as well as non-mutagenic carcinogens, including carcinogens that are not detectable by the Ames assay or by various other short-term tests, such as safrole, urethane, ethionine, auramine, methylene chloride, carbon tetrachloride, cadmium sulfate, aniline, dimethylhydrazine, aminotriazole, acetamide, thiourea and DDE. The present report shows the data for these as well as for additional agents, their response profiles with different concentrations of the agents and the protocol for the DEL system.