Synthesis and properties of a new fluorescent bicyclic 4-N-carbamoyldeoxycytidine derivative
Synthesis and properties of a new fluorescent bicyclic 4-N-carbamoyldeoxycytidine derivative
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DOI：
10.1021/ol053125n
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发表时间：
2006-04-13
期刊：
影响因子：
5.2
通讯作者：
Sekine, M
中科院分区：
文献类型：
--
作者：
Miyata, K;Tamamushi, R;Sekine, M
合成了一种新的双环4-N-氨甲酰脱氧胞苷衍生物（1，dC（hpp））。用T-m分析法研究了含dC（hpp）的寡核苷酸的杂交特性。发现dC（hpp）不仅与互补的鸟嘌呤碱基形成稳定的碱基对，而且与腺嘌呤碱基形成稳定的碱基对。有趣的是，只有当dC（hpp）-dG碱基对形成时，dC（hpp）的荧光才被抑制。
A bicyclic 4-N-carbamoyideoxycytidine derivative (1, dC(hpp)) geometrically locked was synthesized as a new fluorescent nucleobase. The hybridization properties of oligodeoxynucleotides containing dC(hpp) were investigated by use of T-m analysis. It was found that dC(hpp) forms stable base pairs not only with the complementary guanine base, but also with the adenine base. Interestingly, the fluorescence of dC(hpp) was suppressed only when a dC(hpp)-dG base pair was formed.