Multifunctional darkfield microscopy using an axicon

Multifunctional darkfield microscopy using an axicon
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使用轴锥镜的多功能暗视野显微镜

DOI:
10.1117/1.2960019
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发表时间:
2008-07-01
影响因子:
3.5
通讯作者:
Yao, Baoli
Yao, Baoli
中科院分区:
医学3区
文献类型:
--
作者:
Lei, Ming;Yao, Baoli

文献摘要

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We present a multifunctional darkfield microscopy using an axicon. It combines the functions of a darkfield microscope, fluorescence microscope, and microspectrophotometer in one platform. The advantage of the system over conventional darkfield microscopy includes the high transmittance of the illuminating flux, the high contrast of the image, and the convenient toggle between darkfield and brightfield microscopy. Examples of dark, bright, and fluorescent micrographs as well as concerned spectra of microsized specimens implemented in this apparatus are demonstrated. (C) 2008 Society of Photo-Optical Instrumentation Engineers.