Spreading and enhanced motility of human keratinocytes on fibronectin.
Spreading and enhanced motility of human keratinocytes on fibronectin.
复制标题
人类角质形成细胞在纤连蛋白上的扩散和运动性增强。
DOI：
10.1111/1523-1747.ep12276531
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发表时间：
1985
期刊：
影响因子：
--
通讯作者：
Woodley,DT
中科院分区：
文献类型：
--
作者：
O'Keefe,EJ;PayneJr,RE;Russell,N;Woodley,DT
可溶性人血浆纤维连接蛋白或I型或IV型胶原蛋白，当与组织培养塑料培养皿预孵育时，是培养的人角质形成细胞的有效扩散剂，并以时间和浓度依赖的方式增加扩散。抗纤连蛋白抑制了在纤连蛋白上的扩散，但不抑制在IV型胶原上的扩散；因此，纤维连接蛋白对角质形成细胞产生的天然基质的扩散活性的贡献不能用抗纤维连接蛋白来确定。纤维蛋白介导高（1.1 mM）和低（0.1 mM） Ca++浓度下的扩散，环己亚胺不改变扩散。角化细胞沉积的不溶性纤维连接蛋白与颗粒金盐的吞噬动力学轨迹相关，加入纤维连接蛋白以及I型胶原和IV型胶原可增强角化细胞的运动性。这些研究表明，纤维连接蛋白的产生和对纤维连接蛋白的反应在成纤维细胞和角质形成细胞中是相似的，并表明纤维连接蛋白可以作为角质形成细胞的基质因子。
Soluble human plasma fibronectin or collagen types I or IV, when preincubated with tissue culture plastic dishes, were effective spreading agents for cultured human keratinocytes and increased spreading in a time and concentration-dependent manner. Spreading on fibronectin, but not on type IV collagen, was inhibited by antifibroneetin; therefore, the contribution of fibronectin to the spreading activity of the natural matrix produced by keratinocytes could not be determined using antifibronectin. Fibroneetin mediated spreading at both high (1.1 mM) and low (0.1 mM) Ca++concentrations, and spreading was not altered by cycloheximide. Insoluble fibronectin deposited by keratinocytes correlated with phagokinetic tracks on particulate gold salts, and added fibronectin, as well as type I collagen and type IV collagen, enhanced motility of keratinocytes. These studies show that production of fibronectin and responsiveness to it are similar in fibroblasts and keratinocytes and demonstrate that fibronectin can act as a matrix factor for keratinocytes.