Ions released from a S-PRG filler induces oxidative stress in Candida albicans inhibiting its growth and pathogenicity
Ions released from a S-PRG filler induces oxidative stress in Candida albicans inhibiting its growth and pathogenicity
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DOI：
10.1007/s12192-018-0922-1
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发表时间：
2018-11-01
影响因子：
3.8
通讯作者：
Imai, Kenichi
中科院分区：
文献类型：
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作者：
Tamura, Muneaki;Cueno, Marni E.;Imai, Kenichi
白色念珠菌引起机会性真菌感染，通常隐藏在更占优势的细菌中，在体内不表现出高致病性。在老年人中，由于免疫功能减退导致宿主对病原体的抵抗力降低，口腔念珠菌病更容易发展，往往导致全身念珠菌病。表面预反应玻璃离子聚合物填料（S-PRG填料）是一种离子释放功能性生物活性玻璃，可以释放和补充六种离子，从而增强牙齿结构，抑制龋齿引起的脱矿，抑制牙菌斑积聚。然而，其对白色念珠菌的作用从未被阐明。本文研究了S-PRG填料释放的离子对白色念珠菌的影响。结果表明，含有释放离子（ELIS）的提取液降低了白色念珠菌体内过氧化氢的含量和过氧化氢酶的活性。此外，ELIS的存在对白色念珠菌的影响有：(1)抑制真菌生长和生物膜的形成，(2)阻止假牙基托树脂的粘附，(3)抑制二态转化，(4)阻碍分泌天冬氨酸蛋白酶的能力。综上所述，我们的研究结果表明ELIS诱导白色念珠菌氧化应激并抑制其生长和致病性。在这方面，我们建议ELIS有潜力在临床上用于帮助预防老年人口腔念珠菌病的发病和抑制。
Candida albicans causes opportunistic fungal infections usually hidden among more dominant bacteria and does not exhibit high pathogenicity in vivo. Among the elderly, due to reduced host resistance to pathogens attributable to immunoscenesence, oral candidiasis is more likely to develop often leading to systemic candidiasis. Surface pre-reacted glass ionomer filler (S-PRG filler) is an ion-releasing functional bioactive glass that can release and recharge six ions which in turn strengthens tooth structure, inhibits demineralization arising from dental caries, and suppresses dental plaque accumulation. However, its effects on C. albicans have never been elucidated. Here, we evaluated the effects of ion released from S-PRG filler on C. albicans. Results show that extraction liquids containing released ions (ELIS) decreased the amount of hydrogen peroxide and catalase activity in C. albicans. Moreover, ELIS presence was found to affect C. albicans: (1) suppression of fungal growth and biofilm formation, (2) prevent adherence to denture base resin, (3) inhibit dimorphism conversion, and (4) hinder the capability to produce secreted aspartyl proteinase. Taken together, our findings suggest that ELIS induces oxidative stress in C. albicans and suppresses its growth and pathogenicity. In this regard, we propose that ELIS has the potential to be clinically used to help prevent the onset and inhibition of oral candidiasis among the elderly population.