Strong and selective binding of amiloride to thymine base opposite AP sites in DNA duplexes: simultaneous binding to DNA phosphate backbone.
Strong and selective binding of amiloride to thymine base opposite AP sites in DNA duplexes: simultaneous binding to DNA phosphate backbone.
复制标题
DOI：
10.1039/b516575j
复制
发表时间：
2006-03
影响因子：
4.9
通讯作者：
Chunxia Zhao;Qing Dai;T. Seino;Ying Cui;S. Nishizawa;N. Teramae
中科院分区：
文献类型：
--
作者：
Chunxia Zhao;Qing Dai;T. Seino;Ying Cui;S. Nishizawa;N. Teramae
阿米洛利（N-脒基-3，5-二氨基-6-氯-吡嗪甲酰胺盐酸盐）具有两组适合于靶核苷酸和磷酸二酯DNA骨架的氢键形成位点，通过所述氢键形成位点和磷酸二酯DNA骨架，可以高选择性和亲和力识别DNA双链体中与脱碱基位点相对的胸腺嘧啶碱基，并且其适用于PCR扩增产物的胸腺嘧啶相关SNP（单核苷酸多态性）的荧光检测。
Amiloride (N-amidino-3,5-diamino-6-chloro-pyrazinecarboxamide hydrochloride) has two sets of hydrogen-bond forming sites suitable for target nucleotides and the phosphodiester DNA backbone by which a thymine base opposite an abasic site in DNA duplexes can be recognized with high selectivity and affinity, and it is applicable to the fluorescence detection of thymidine-related SNPs (single-nucleotide polymorphisms) of PCR amplification products.