A cross-platform freeware tool for digital reconstruction of neuronal arborizations from image stacks
A cross-platform freeware tool for digital reconstruction of neuronal arborizations from image stacks
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DOI：
10.1385/ni:3:4:343
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发表时间：
2005-01-01
期刊：
影响因子：
3
通讯作者：
Ascoli, GA
中科院分区：
文献类型：
--
作者：
Brown, KM;Donohue, DE;Ascoli, GA
神经元分支的数字化重建是细胞神经解剖学定量研究的重要一步。在这个过程中，通过使用专门的计算机软件，通过显微镜成像的神经突起被半手动追踪，并被表示为分支圆柱体(或截断圆锥体)的二叉树。这种形式的重建文件是高效和简约的，并且允许广泛的形态测量分析以及电生理学的生物物理模型的实现。在这里，我们描述Neuron_Morpho，这是一个流行的Java应用程序ImageJ的插件，它协调从图像堆栈进行神经元的数字重建。Neuron_Morpho的可执行文件和代码都是免费分发的((www.maths.soton.ac.uk/staff/D‘Alessandro/morpho或www.krasnow.gmu.edu/L-Neuron)，并与所有主要的计算机平台(包括Windows、Mac和LINUX)兼容。我们对Neuron_Morpho进行了测试，方法是分别从代表不同脑区(海马和小脑)、神经炎类型(锥体细胞树突和Olivar轴突投射终末)和标记方法(快速高尔基体浸渍和顺行葡聚糖胺)的两个准备材料中重建两个神经元，并将结果与用标准商业系统重建的相同细胞的形态进行定量比较。神经灵敏。在分析的众多形态测量指标中，没有一项显示两个重建系统之间有任何显著或系统的差异。
Digital reconstruction of neuronal arborizations is an important step in the quantitative investigation of cellular neuroanatomy. In this process, neurites imaged by microscopy are semi-manually traced through the use of specialized computer software and represented as binary trees of branching cylinders (or truncated cones). Such form of the reconstruction files is efficient and parsimonious, and allows extensive morphometric analysis as well as the implementation of biophysical models of electrophysiology. Here, we describe Neuron_Morpho, a plug in for the popular Java application ImageJ that mediates the digital reconstruction of neurons from image stacks. Both the executable and code of Neuron_Morpho are freely distributed (www.maths.soton.ac.uk/staff/D'Alessandro/morpho or www.krasnow.gmu.edu/L-Neuron), and are compatible with all major computer platforms (including Windows, Mac, and Linux). We tested Neuron_Morpho by reconstructuring two neurons from each of the two preparations representing different brain areas (hippocampus and cerebellum), neuritic type (pyramidal cell dendrites and olivar axonal projection terminals), and labeling method (rapid Golgi impregnation and anterograde dextran amine), and quantitatively comparing the resulting morphologies to those of the same cells reconstructed with the standard commercial system. Neurolucida. None of the numerous morphometric measures that were analyzed displayed any significant or systematic difference between the two reconstructing systems.