Novel lanthanide-polymer complexes for dye-free dual modal probes for MRI and fluorescence imaging
Novel lanthanide-polymer complexes for dye-free dual modal probes for MRI and fluorescence imaging
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用于 MRI 和荧光成像的无染料双模态探针的新型镧系元素聚合物复合物
DOI：
10.1039/c5py01011j
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发表时间：
2015-01-01
影响因子：
4.6
通讯作者：
Sun, Jihong
中科院分区：
文献类型：
--
作者：
Cao, Fangyi;Huang, Tongcun;Sun, Jihong
高分辨率成像是肿瘤治疗和分期的有力技术。利用镧系杂化纳米粒子作为探针进行双峰成像，由于其特有的物理性质而受到越来越多的关注。本文报道了一种新型的水溶性两亲嵌段共聚物胶束，它具有二酮悬垂物螯合钆（Gd3+）和铕（Eu3+）阳离子，可作为磁共振成像（MRI）和荧光成像的双重探针，用于临床检测。Gd3+和Eu3+混合胶束尺寸分布均匀，流体动力直径在80 ~ 220 nm之间可调。大分子二酮配体提高了Gd3+的弛豫速率，有效地增敏了Eu3+的发光。在Gd3+的共掺杂下，Eu3+配合物表现出长寿命的发射和大的Stokes位移。在350和405 nm的激发范围内，观察到波长为614 nm的强红光。细胞摄取实验显示MCF-7细胞具有良好的生物相容性和快速的摄取能力。这种强大的双峰探针在肿瘤的早期诊断、手术前计划和术中荧光引导手术中有很好的应用前景。
High-resolution imaging is a powerful technique in theranostics and staging of tumors. Dual-modal imaging using lanthanide hybrid nanoparticles as probes has been attracting increasing attention due to their specific and intrinsic physical properties. The present work reports a novel water-soluble micelle of an amphiphilic block copolymer with diketone pendants chelating gadolinium (Gd3+) and europium (Eu3+) cations as a dual probe for magnetic resonance imaging (MRI) and fluorescence imaging for clinical detection. The Gd3+ and Eu3+ hybrid micelles have homogeneous size distribution and hydrodynamic diameters tunable between 80 and 220 nm. The macromolecular diketone ligand enhances the relaxation rate of Gd3+ and sensitizes Eu3+ luminescence efficiently. Further sensitized by the co-doped Gd3+, the Eu3+ complex exhibits a long-lived emission with a large Stokes shift. A strong red light at a wavelength of 614 nm is observed with an excitation in the range of 350 and 405 nm. Cellular uptake experiments reveal excellent biocompatibility and rapid uptake of the nanoparticles by MCF-7 cells. The robust dual-modal probes are promising in applications for early diagnosis of tumors, presurgical planning, and intraoperative fluorescence-guided surgery.