Amplifications of both c-Ki-ras with a point mutation and c-myc in a primary pancreatic cancer and its metastatic tumors in lymph nodes.
Amplifications of both c-Ki-ras with a point mutation and c-myc in a primary pancreatic cancer and its metastatic tumors in lymph nodes.
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原发性胰腺癌及其淋巴结转移肿瘤中具有点突变的 c-Ki-ras 和 c-myc 的扩增。
DOI：
10.20772/cancersci1985.77.4_370
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发表时间：
1986
期刊：
影响因子：
--
通讯作者：
T. Sugimura
中科院分区：
文献类型：
--
作者：
H. Yamada;H. Sakamoto;M. Taira;S. Nishimura;Y. Shimosato;M. Terada;T. Sugimura
在一淋巴结转移性胰腺癌的DNA中发现c-ki-ras基因被激活，并在第12位密码子发生点突变(GGT到CGT)，导致精氨酸取代甘氨酸。通过SacI酶切限制性内切酶长度多态性的方法，我们能够证明c-ki-ras基因在原发灶和淋巴结转移灶中存在相同的点突变。患者正常脾的DNA没有这种类型的点突变。在原发灶和转移灶中，c-ki-ras基因和c-myc基因的扩增倍数分别为3~6倍和50倍，提示该基因的点突变发生在肿瘤发生的较早阶段，在基因扩增之前。这是在活体人类原发和转移性肿瘤中c-ki-ras基因扩增伴随c-myc基因扩增的首次明确证据。
Activated c-Ki-ras with a point mutation (GGT to CGT) at codon 12, resulting in the substitution of arginine for glycine, was found in DNA from metastatic pancreatic adenocarcinoma in a lymph node. By means of restriction endonuclease length polymorphism with SacI digestion, we were able to demonstrate that the same point mutation of c-Ki-ras was present in the primary tumor and in metastases in lymph nodes. DNA from the normal spleen of the patient did not have this type of point mutation. Moreover, amplifications of 3- to 6-fold of the activated c-Ki-ras and 50-fold of c-myc were found in the primary tumor and the metastases in the two lymph nodes, indicating that point mutation had occurred at a relatively early stage of the tumor development, before amplification of the gene. This is the first clear demonstration of amplification of activated c-Ki-ras accompanied by amplification of c-myc in both primary and metastatic human tumors in vivo.
五种人类乳腺癌细胞系之一中 c-myc 癌基因的扩增。
影响因子：
11.2
作者：
Kozbor,D;Croce,CM
通讯作者：
Croce,CM
人类 c-K-ras 癌基因的激活。
影响因子：
14.9
作者：
Yamamoto,F;Perucho,M
通讯作者：
Perucho,M