Isolation, characterization, and chromosomal localization of mouse and human COUP-TF I and II genes.
Isolation, characterization, and chromosomal localization of mouse and human COUP-TF I and II genes.
复制标题
小鼠和人类 COUP-TF I 和 II 基因的分离、表征和染色体定位。
DOI：
10.1006/geno.1995.1237
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发表时间：
1995
期刊：
影响因子：
4.4
通讯作者：
Tsai,SY
中科院分区：
文献类型：
--
作者：
Qiu,Y;Krishnan,V;Zeng,Z;Gilbert,DJ;Copeland,NG;Gibson,L;Yang-Feng,T;Jenkins,NA;Tsai,MJ;Tsai,SY
鸡卵清蛋白上游启动子转录因子 (COUP-TF) 是类固醇/甲状腺激素受体超家族的孤儿成员。 COUP-TF 同源物已在从果蝇到人类的许多物种中被克隆。 COUP-TF 的蛋白质序列在物种间高度同源，表明功能保守。两个 COUP-TF 基因已从人类中克隆出来，并且它们的基因组结构已被表征。为了确定人类和小鼠之间的基因组组织是否保守，我们分离了两个小鼠COUP-TF基因（I和III）并表征了它们的基因组结构。这两个基因具有与人类对应基因相似的相对简单的结构。此外，我们将小鼠COUP-TF I映射到13号染色体的远端区域，将COUP-TF II映射到7号染色体的中央区域。此外，我们将人类COUP-TF I映射到5号染色体的5q14，将COUP-TF II映射到5号染色体的5q14。 15号染色体15q26。结果表明COUP-TF基因位于小鼠和人类同线的染色体区域。
Chicken ovalbumin upstream promoter transcription factors (COUP-TFs) are orphan members of the steroid/thyroid hormone receptor superfamily. COUP-TF homologues have been cloned in many species, from Drosophila to human. The protein sequences of COUP-TFs are highly homologous across species, suggesting functional conservation. Two COUP-TF genes have been cloned from human, and their genomic organizations have been characterized. To determine whether the genomic organization is conserved between human and mouse, we isolated two mouse COUP-TF genes (I and III and characterized their genomic structures. Both genes have relatively simple structures that are similar to those of their human counterparts. In addition, we mapped mouse COUP-TF I to the distal region of chromosome 13 and COUP-TF II to the central region of chromosome 7. Furthermore, we mapped human COUP-TF I to 5q14 of chromosome 5 and COUP-TF II to 15q26 of chromosome 15. The results demonstrate that COUP-TF genes are located in chromosomal regions that are syntenic between mouse and human.