Synchronous fluorescence measurement of enrofloxacin in the pharmaceutical formulation and its residue in milks based on the yttrium (III)-perturbed luminescence.
Synchronous fluorescence measurement of enrofloxacin in the pharmaceutical formulation and its residue in milks based on the yttrium (III)-perturbed luminescence.
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DOI：
10.1016/j.talanta.2010.07.082
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发表时间：
2010-10
期刊：
影响因子：
6.1
通讯作者：
C. Tong;Xiajun Zhuo;Wei-ping Liu;Jianmin Wu
中科院分区：
文献类型：
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作者：
C. Tong;Xiajun Zhuo;Wei-ping Liu;Jianmin Wu
提出了一种基于钇(III)微扰发光的简单、快速、灵敏的同步荧光方法，用于测定药物制剂中的恩诺沙星(ENRO)及其在牛奶中的残留量。当ENRO溶液中添加Y3+时，ENRO的荧光显着增强。本发明采用同步荧光技术测定牛奶中微量ENRO残留量。在1cm石英池中测量系统的同步荧光强度，激发波长为328nm，Δλ=80nm。在1.0×10−9~2.0×10−6molL−1（r2=0.9992）范围内，荧光强度与ENRO浓度呈良好的线性关系。该方法的检测限(LOD)低至3.0×10−10molL−1(S/N=3)。该方法的选择性也很好。常见的金属离子、稀土离子和一些药物通常与ENRO一起使用，在实际情况下不会干扰ENRO的测定。该方法可用于测定牛奶中的 ENRO 残留量，加标牛奶中测定的定量限 (LOQ) 估计为 2.8×10−8molL−1(10μgL−1)。此外，该方法还可用于快速筛查判断牛奶中ENRO残留是否超过最低风险水平（MRL）。此外，还详细讨论了荧光增强的机制。
A simple, rapid and sensitive synchronous fluorescence method is put forward for the determination of enrofloxacin (ENRO) in the pharmaceutical formulation and its residue in milk based on the yttrium (III)-perturbed luminescence. When Y3+is added into the ENRO solution, the fluorescence of ENRO is significantly enhanced. The synchronous fluorescence technology is employed in the method to determine trace amount of ENRO residue in milks. The synchronous fluorescence intensity of the system is measured in a 1-cm quartz cell with excitation wavelength of 328nm, Δλ=80nm. A good linear relationship between the fluorescence intensity and the ENRO concentration is obtained in the range of 1.0×10−9to 2.0×10−6molL−1(r2=0.9992). The limit of detection (LOD) of this method attains as low as 3.0×10−10molL−1(S/N=3). The selectivity of this method is also very good. Common metal ions, rare-earth ions and some pharmaceuticals, which are usually used together with ENRO, do not interfere with the determination of ENRO under the actual conditions. The proposed method can be applied to determine ENRO residue in milks, and limit of quantification (LOQ) determined in the spiked milk is estimated to be 2.8×10−8molL−1(10μgL−1). Moreover, this method can be used as a rapid screening for judging whether the ENRO residues in milks exceed Minimal Risk Levels (MRLs) or not. In addition, the mechanism of the fluorescence enhancement is also discussed in detail.