Electroacupuncture inhibits visceral pain via adenosine receptors in mice with inflammatory bowel disease
Electroacupuncture inhibits visceral pain via adenosine receptors in mice with inflammatory bowel disease
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DOI：
10.1007/s11302-019-09655-4
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发表时间：
2019-06-01
影响因子：
3.5
通讯作者：
Li, Man
中科院分区：
文献类型：
--
作者：
Hou, Tengfei;Xiang, Hongchun;Li, Man
探讨外周腺苷受体参与电针对炎症性肠病（IBD）小鼠内脏痛的影响。采用2，4，6-三硝基苯磺酸（TNBS）诱导内脏痛模型。电针治疗组于注射TNBS后1d电针双侧大肠俞穴（1 mA，2 Hz，30 min），每日1次，连续7天。用Von Frey细丝测量机械痛阈。Western blot检测结肠组织腺苷1受体（A1 R）、腺苷2a受体（A2 aR）、腺苷2b受体（A2 bR）、腺苷3受体（A3 R）、P物质（SP）和白细胞介素1 β（IL-1 β）的蛋白表达水平。电针可明显改善IBD小鼠的疾病相关指标，降低IBD小鼠结肠组织中SP和IL-1 β的表达。电针可增加结肠组织中A1 R、A2 aR和A3 R的表达，降低A2 bR的表达。此外，腺苷受体拮抗剂的管理影响EA的效果。电针可通过调节外周A1、A2 a、A2 b和A3受体抑制炎症因子SP和IL-1 β的表达，从而抑制IBD小鼠内脏痛。
To investigate the involvement of peripheral adenosine receptors in the effect of electroacupuncture (EA) on visceral pain in mice with inflammatory bowel disease (IBD). 2,4,6-Trinitrobenzene sulfonic acid (TNBS) was used to induce the visceral pain model. EA (1mA, 2Hz, 30min) treatment was applied to bilateral acupoints Dachangshu (BL25) 1day after TNBS injection once daily for 7 consecutive days. Von Frey filaments were used to measure the mechanical pain threshold. Western blot was used to detect the protein expression levels of adenosine 1 receptor (A1R), adenosine 2a receptor (A2aR), adenosine 2b receptor (A2bR), adenosine 3 receptor (A3R), substance P (SP), and interleukin 1 beta (IL-1 beta) in colon tissue. EA significantly ameliorated the disease-related indices and reduced the expression of SP and IL-1 beta in the colon tissues of mice with IBD. EA increased the expression of A1R, A2aR, and A3R and decreased the expression of A2bR in the colon tissue. Furthermore, the administration of adenosine receptor antagonists influenced the effect of EA. EA can inhibit the expression of the inflammatory factors SP and IL-1 beta by regulating peripheral A1, A2a, A2b, and A3 receptors, thus inhibiting visceral pain in IBD mice.