Characterization of an in vitro model for evaluating the interface between skin and percutaneous biomaterials
Characterization of an in vitro model for evaluating the interface between skin and percutaneous biomaterials
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DOI：
10.1111/j.1743-6109.2006.00138.x
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发表时间：
2006-07-01
影响因子：
2.9
通讯作者：
Olerud, John E.
中科院分区：
文献类型：
--
作者：
Fukano, Yuko;Knowles, Negar G.;Olerud, John E.
经皮器械在医学中起着至关重要的作用;然而，它们通常与显著的感染风险相关。避免感染的一种方法是通过促进皮肤细胞附着来治愈设备周围的伤口。我们使用了两种体外试验模型来评估皮肤对聚（甲基丙烯酸2-羟乙酯）（聚（HEMA））的反应。一种方法是使用细胞粘附试验来测试聚（HEMA）的表面改性的效果，第二种方法是使用植入多孔聚（HEMA）棒（20 μ m孔）的新生儿包皮活检的器官培养系统来评估皮肤/聚（HEMA）界面。使用1，1 '-羰基二咪唑（CDI）对聚（HEMA）进行表面改性，可增强角质形成细胞、成纤维细胞和内皮细胞的粘附。在器官培养模型中的角质形成细胞不仅保持功能和结构上的可行性，通过免疫组织化学和电子显微镜观察，但迁移到孔的CDI修饰的聚（HEMA）棒。在非CDI修饰的聚（HEMA）中未观察到生物整合。沿着聚（HEMA）/皮肤界面观察到层粘连蛋白5免疫染色，其图案类似于牙齿的结合上皮，皮肤和牙齿之间独特的天然界面，用作细菌屏障。对用于动物植入研究的生物材料进行体外系统评价既节省成本又节省时间。
Percutaneous devices play an essential role in medicine; however, they are often associated with a significant risk of infection. One approach to circumvent infection would be to heal the wound around the devices by promoting skin cell attachment. We used two in vitro assay models to evaluate cutaneous response to poly(2-hydoxyethyl methacrylate) (poly(HEMA)). One approach was to use a cell adhesion assay to test the effects of surface modification of poly(HEMA), and the second used an organ culture system of newborn foreskin biopsies implanted with porous poly(HEMA) rods (20 mu m pores) to evaluate the skin/poly(HEMA) interface. Surface modification of poly(HEMA) using 1,1'-carbonyldiimidazole (CDI) enhanced keratinocyte, fibroblast, and endothelial cell adhesion. Keratinocytes in the organ culture model not only remained functionally and structurally viable as observed by immunohistochemistry and electron microscopy, but migrated into the pores of CDI-modified poly(HEMA) rods. No biointegration was seen in the non-CDI-modified poly(HEMA). Laminin 5 immunostaining was seen along the poly(HEMA)/skin interface in a pattern resembling the junctional epithelium of the tooth, the unique natural interface between the skin and tooth that serves as a barrier to bacteria. In vitro systematic evaluation of biomaterials for use in animal implant studies is both cost effective and time efficient.