Knockdown of Bcl-3 Inhibits Cell Growth and Induces DNA Damage in HTLV-1-infected Cells)
Knockdown of Bcl-3 Inhibits Cell Growth and Induces DNA Damage in HTLV-1-infected Cells)
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Bcl-3 的敲低会抑制细胞生长并诱导 HTLV-1 感染细胞中的 DNA 损伤）
DOI：
10.7314/apjcp.2013.14.1.405
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发表时间：
2013-01-01
影响因子：
--
通讯作者：
Wang, Hui
中科院分区：
文献类型：
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作者：
Gao, Cai;Wang, Xia;Wang, Hui
肿瘤蛋白Bcl-3被认为是I kappa B家族的一个不寻常的成员，因为它可以刺激和抑制NF-kappa B的激活。异常的Bcl-3导致细胞增殖和存活增加，提示在许多htlv -1感染的T细胞系和ATL细胞中观察到Bcl-3水平升高对恶性潜能的贡献。为了研究Bcl-3在htlv -1感染细胞中的具体作用，我们使用shRNA敲除Bcl-3的表达，然后检测其对DNA损伤和细胞增殖以及NF-kappa B激活的影响。HTLV-1编码的蛋白Tax促进Bcl-3的表达和核易位。在htlv -1感染细胞中，Bcl-3敲低明显诱导DNA损伤。当Bcl-3表达降低时，htlv -1感染或Tax阳性细胞的细胞生长和NF-kappa B活化均降低。总之，我们的研究结果揭示了Bcl-3在htlv -1感染细胞的DNA稳定、生长和NF-kappa B激活方面的积极作用。
Oncoprotein Bcl-3 is perceived as an unusual member of I kappa B family since it can both stimulate and suppress NF-kappa B activation. Aberrant Bcl-3 results in increased cell proliferation and survival, suggesting a contribution to malignant potential and elevated levels of Bcl-3 have been observed in many HTLV-1-infected T cell lines and ATL cells. To investigate the specific roles of Bcl-3 in HTLV-1-infected cells, we knocked down Bcl-3 expression using shRNA and then examined the consequences with regard to DNA damage and cell proliferation, as well as NF-kappa B activation. The HTLV-1 encoded protein Tax promotes Bcl-3 expression and nuclear translocation. In HTLV-1-infected cells, Bcl-3 knockdown obviously induced DNA damage. Cell growth and NF-kappa B activation were reduced in HTLV-1-infected or Tax positive cells when Bcl-3 expression was decreased. Together, our results revealed positive roles of Bcl-3 in DNA stabilization, growth and NF-kappa B activation in HTLV-1-infected cells.