Process for the preparation of pathogen-inactivated RBC concentrates by using PEN110 chemistry: preclinical studies.
Process for the preparation of pathogen-inactivated RBC concentrates by using PEN110 chemistry: preclinical studies.
复制标题
使用 PEN110 化学制备病原体灭活红细胞浓缩物的工艺：临床前研究。
DOI：
10.1046/j.1537-2995.2002.00020.x
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发表时间：
2002
期刊：
影响因子：
2.9
通讯作者：
Chapman,John
中科院分区：
文献类型：
--
作者：
Purmal,Andrei;Valeri,CRobert;Dzik,Walter;Pivacek,Linda;Ragno,Gina;Lazo,Aris;Chapman,John
背景：正在使用PEN 110化学（INACTINE，V.I.技术）。本研究的目的是表征使用PEN 110工艺制备的RBC的质量，并测量针对两种virus.Study设计和方法实现的杀病毒效果：病毒学和RBC研究是用0.1%（vol/vol）PEN 110在22°C下处理6小时的标准RBC单位进行的。使用生物化学和表型变量评估PEN 110处理的人RBC的质量。采用双标记51 Cr/125 I方法研究了经PEN 110处理的狒狒红细胞的体内活力。结果：观察到每mL牛病毒性腹泻病毒（一种包膜RNA病毒）和猪细小病毒（一种无包膜DNA病毒）的组织培养感染剂量超过5 log 50%时，感染滴度降低。储存42天后，RBC溶血小于1%，未观察到RBC抗原的变化。PEN 110处理的狒狒RBC的体内活力与对照相比没有变化。结论：通过使用PEN 110工艺制备RBC实现了两种不同病毒的显著病毒减少，而不会对RBC造成不良影响。这一过程似乎是一个有希望的方法，因此值得进一步研究。
BACKGROUND:A pathogen‐inactivation process for RBC concentrates is being developed by using PEN110 chemistry (INACTINE, V.I. Technologies). The objective of this study was to characterize the quality of RBCs prepared by using the PEN110 process and to measure the virucidal effect achieved against two viruses.STUDY DESIGN AND METHODS:Virology and RBC studies were conducted with standard RBC units treated with 0.1‐percent (vol/vol) PEN110 at 22°C for 6 hours. The quality of PEN110‐treated human RBCs was assessed with biochemical and phenotypic variables. The in vivo viability of PEN110‐treated RBCs in baboons was studied with the double‐label51Cr/125I method.RESULTS:Decreases in infectious titer by inactivation of greater than a 5 log 50‐percent tissue culture infectious doses per mL of bovine viral diarrhea virus (an enveloped RNA virus) and porcine parvovirus (a nonenveloped DNA virus) was observed. RBC hemolysis was less than 1 percent after 42 days of storage, and no changes in RBC antigens were observed. The in vivo viability of PEN110‐treated baboon RBCs was unchanged from control.CONCLUSION:The preparation of RBCs by using the PEN110 process achieved a significant viral reduction of two diverse viruses without causing adverse effects to the RBCs. The process appears to be a promising approach, thus justifying further study.
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影响因子：
1.9
作者：
Paul E. Cullum;David E. Frost;Timothy B. Newland;Thomas M. Keane;William J. Ehler
通讯作者：
William J. Ehler
DOI：
--
发表时间：
1981
期刊：
The Journal of the American Dental Association (1939)
影响因子：
--
作者：
H. Kwon;D. Waite;F. Stickel;T. Chisholm;F. McParland
通讯作者：
F. McParland
影响因子：
3
作者：
MARKS, SC;CAHILL, DR
通讯作者：
CAHILL, DR
DOI：
10.1111/j.1600-0714.1987.tb02060.x
发表时间：
2007-07
期刊：
Journal of oral pathology
影响因子：
--
作者：
S. Marks,;D. R. Cahill
通讯作者：
S. Marks,;D. R. Cahill
DOI：
--
发表时间：
1974
期刊：
Laboratory animal science
影响因子：
--
作者：
E. Berman
通讯作者：
E. Berman