Real-time deformability cytometry: on-the-fly cell mechanical phenotyping
Real-time deformability cytometry: on-the-fly cell mechanical phenotyping
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DOI：
10.1038/nmeth.3281
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发表时间：
2015-03-01
期刊：
影响因子：
48
通讯作者：
Guck, Jochen
中科院分区：
文献类型：
--
作者：
Otto, Oliver;Rosendahl, Philipp;Guck, Jochen
我们介绍了实时变形能力细胞仪(RT-DC)，用于分析大于100个细胞/S的大群体(100,000个细胞)的连续细胞力学特征。RT-DC对细胞骨架的变化很敏感，可以区分细胞周期的不同阶段，跟踪干细胞向不同谱系的分化，并通过其机械指纹识别全血中的细胞群体。这项技术为流式细胞术增加了一个新的无标记维度，在生物学、生物技术和医学中有着广泛的应用。
We introduce real-time deformability cytometry (RT-DC) for continuous cell mechanical characterization of large populations (> 100,000 cells) with analysis rates greater than 100 cells/s. RT-DC is sensitive to cytoskeletal alterations and can distinguish cell-cycle phases, track stem cell differentiation into distinct lineages and identify cell populations in whole blood by their mechanical fingerprints. This technique adds a new marker-free dimension to flow cytometry with diverse applications in biology, biotechnology and medicine.