Duration of ultrasound-mediated enhanced plasma membrane permeability
Duration of ultrasound-mediated enhanced plasma membrane permeability
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DOI：
10.1016/j.ijpharm.2014.12.013
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发表时间：
2015-03-30
影响因子：
5.8
通讯作者：
Bos, Clemens
中科院分区：
文献类型：
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作者：
Lammertink, Bart;Deckers, Roel;Bos, Clemens
超声空化可以通过瞬时增加细胞膜的通透性来增强药物的细胞内递送。这种增加的渗透性的持续时间，称为时间窗口，尚未完全阐明。在这项研究中，时间窗口进行了系统的研究，使用内皮细胞和两个乳腺癌细胞系。在超声处理后，在SonoVue（TM）微泡存在下，在HUVEC、MDA-MB-468和4 T1细胞中测量模型药物摄取作为时间的函数。此外，在MDA-MB-468细胞中改变US压力幅度以研究其对时间窗的影响。HUVEC和MDA-MB-468细胞的细胞膜通透性在超声处理后1-2 h内恢复到对照水平，而4 T1细胞需要3 h以上。超声压力影响膜通透性增加的细胞数量，以及MDA-MB-468细胞的时间窗。该研究表明，在这里使用的细胞系和US压力之间，膜渗透性增加的持续时间不同。然而，在超声处理后1-3小时，所有这些都是一致的。(C)© 2014 Elsevier B. V.保留所有权利。
Ultrasound (US) induced cavitation can be used to enhance the intracellular delivery of drugs by transiently increasing the cell membrane permeability. The duration of this increased permeability, termed temporal window, has not been fully elucidated. In this study, the temporal window was investigated systematically using an endothelial-and two breast cancer cell lines. Model drug uptake was measured as a function of time after sonication, in the presence of SonoVue (TM) microbubbles, in HUVEC, MDA-MB-468 and 4T1 cells. In addition, US pressure amplitude was varied in MDA-MB-468 cells to investigate its effect on the temporal window. Cell membrane permeability of HUVEC and MDA-MB-468 cells returned to control level within 1-2 h post-sonication, while 4T1 cells needed over 3 h. US pressure affected the number of cells with increased membrane permeability, as well as the temporal window in MDA-MB-468 cells. This study shows that the duration of increased membrane permeability differed between the cell lines and US pressures used here. However, all were consistently in the order of 1-3 h after sonication. (C) 2014 Elsevier B.V. All rights reserved.