Preliminary studies on the primary structure of rat liver dihydropteridine reductase.

Preliminary studies on the primary structure of rat liver dihydropteridine reductase.
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大鼠肝脏二氢蝶啶还原酶一级结构的初步研究。

DOI:
10.1016/0006-291x(87)91393-3
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发表时间:
1987
影响因子:
3.1
通讯作者:
Whiteley,JM
Whiteley,JM
中科院分区:
生物学4区
文献类型:
--
作者:
Webber,S;Hural,J;Whiteley,JM

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溴化氰裂解的还原烷基化均质大鼠肝二氢蝶啶还原酶提供了几个肽片段,可通过聚丙烯酰胺电泳,其中6个(CB-1至CB-6)可以单独分离的C8反相HPLC。每一种都通过N-末端氨基酸分析表征,并推导出CB-1、CB-4和CB-6的序列信息。N-末端封闭的全酶被鉴定为焦谷氨酸,C-末端序列通过连续降解获得。
Cyanogen bromide cleavage of reductively alkylated homogeneous rat liver dihydropteridine reductase afforded several peptide fragments identifiable by polyacrylamide electrophoresis of which 6 (CB-1 to CB-6) could be individually isolated by C8reverse phase HPLC. Each was characterised by N-terminal amino acid analysis and sequence information was derived for CB-1, CB-4 and CB-6. The blocked N-terminal of the holoenzyme was identified as pyroglutamate and the C-terminal sequence was obtained by sequential degradation.
DOI: 10.1021/bi00842a005
发表时间: 1968
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DOI: --
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期刊: The Journal of biological chemistry
影响因子: --
作者:
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DOI: 10.1016/0003-9861(86)90432-7
发表时间: 1986
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作者:
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影响因子: --
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