The AC impedance of necturus gallbladder epithelium

The AC impedance of necturus gallbladder epithelium
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油管胆囊上皮的交流阻抗

DOI:
10.1007/bf00582842
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发表时间:
1978
期刊:
Pflügers Archiv
影响因子:
--
通讯作者:
E. Frömter
E. Frömter
中科院分区:
--
文献类型:
--
作者:
E. Schifferdecker;E. Frömter

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The impedance of Necturus gallbladder epithelium was determined using sine wave currents of 1 Hz to 30 kHz. In control Ringer's solution the impedance locus exhibited a simple semicircle with minute shift of the high frequency end along the real axis and a minute depression of the center below the real axis (average 0.9±0.7°). Neglecting the slight suppression, the impedance of 1 cm2 of epithelium can be represented by an electrical analogue consisting of a parallel RC element of 115±26Ω and 5.16±0.9 μF in series with a small resistor of 5.3±1.3Ω. In agreement with experimental results obtained under ionic or osmotic substitutions, the applicability of this simple RC analogue to gallbladder epithelium under control conditions can be explained by the influence of the paracellular shunt and by assuming the time constants of the apical and basal cell membranes to be comparable. Based on these data and on voltage divider measurements obtained with microelectrodes the capacitances of the apical and basal cell membrane can be estimated to be ∼ 7 and ∼ 18 μF/cm2. The latter value agrees well with estimates of the surface folding obtained from electronmicrographs, if the specific cell membrane capacitance is assumed to be ∼ 1 μF/cm2 as in other cell membranes.
运输上皮细胞的核磁共振分析。
DOI: --
发表时间: 1983
期刊: Progress in clinical and biological research
影响因子: --
作者:
Civan,MM
通讯作者: Civan,MM