Oxidative Stress-induced DNA Damage in the Synovial Cells of the Temporomandibular Joint in the Rat
Oxidative Stress-induced DNA Damage in the Synovial Cells of the Temporomandibular Joint in the Rat
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DOI：
10.1177/154405910408300807
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发表时间：
2004-08
影响因子：
7.6
通讯作者：
T. Yamaza;K. F. Masuda;I. Atsuta;K. Nishijima;M. Kido;T. Tanaka
中科院分区：
文献类型：
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作者：
T. Yamaza;K. F. Masuda;I. Atsuta;K. Nishijima;M. Kido;T. Tanaka
滑膜增生是退行性颞下颌关节病的一个特征。然而，TMJ增生进展的机制尚不清楚。基于机械负荷产生的氧化应激导致TMJ滑膜退行性变化的假设，我们研究了高活性物质过氧亚硝酸盐的产生和滑膜中DNA损伤的发生。在大鼠TMJ髁突过度活动后，过氧亚硝基标记物硝基酪氨酸定位于滑膜炎诱导的TMJ中滑膜衬里细胞和成纤维细胞的细胞核和细胞质。DNA单链断裂仅在酶处理后的滑膜细胞的细胞核中发现，而DNA双链断裂未检测到。这些结果表明，髁状突过度运动诱导滑膜细胞的增殖，并表明，氧化应激导致滑膜细胞的DNA损伤后，在TMJ的机械负荷的滑膜增生的进展。
Synovial hyperplasia is a feature of degenerative temporomandibular joint (TMJ) disease. However, the mechanism by which hyperplasia progresses in the TMJ is unknown. Based on the hypothesis that the oxidative stress generated by mechanical loading causes degenerative changes in the TMJ synovium, we investigated the generation of the highly reactive species, peroxynitrite, and the occurrence of DNA damage in the synovium. After condylar hypermobility of rat TMJs, a marker of peroxynitrite, nitrotyrosine, was localized to the nuclei and cytoplasm of the synovial lining cells and fibroblasts in synovitis-induced TMJ. DNA single-strand breaks were found in the nuclei of the synovial cells only after enzyme treatment, whereas DNA double-strand breaks were not detected. These findings indicate that condylar hypermovement induces the proliferation of synovial cells, and suggest that oxidative stress leads to the progression of synovial hyperplasia via DNA damage of the synovial cells in TMJs after mechanical loading.