Altered metabolic regulation owing to gsp1 mutations encoding the nuclear small G protein in Saccharomyces cerevisiae.
Altered metabolic regulation owing to gsp1 mutations encoding the nuclear small G protein in Saccharomyces cerevisiae.
复制标题
酿酒酵母中编码核小 G 蛋白的 gsp1 突变改变了代谢调节。
DOI：
10.1007/s00294-019-01022-5
复制
发表时间：
2020
期刊：
影响因子：
2.5
通讯作者：
Masaya Oki
中科院分区：
文献类型：
--
作者：
Naoyuki Hayashi;Masaya Oki
营养代谢受到调节，以适应环境变化、细胞应激、细胞周期和细胞老化等。这个调控网络由细胞质细胞器和细胞核之间的串扰组成。ras样核小G蛋白Ran在核胞质转运和调控信号传递中起作用。在酵母中，酵母菌的Ran系统中涉及的一些基因是在甘油培养基上生长所必需的。由于编码Ran的gsp1基因突变而导致的生长缺陷是等位基因特异性的。具体来说，在本研究中，gsp1-1894细胞失去了线粒体，并且不能在含有甘油、半乳糖或麦芽糖的培养基上生长。然而，gsp1-1894细胞在高盐培养基（1 M NaCl）中生长较好，gpd1 - lacz表达水平升高。此外，hog1基因的破坏抑制了它们在甘油培养基上的生长缺陷。这些发现表明，gsp1-1894细胞中Hog1激活的改变导致线粒体的丢失和甘油代谢的抑制。高剂量的编码AMPK胞质β亚基的theSIP2DNA进一步抑制了gsp1-1894细胞在半乳糖培养基上的生长缺陷。这表明sp1-1894细胞需要更高的AMPK细胞质活性来利用替代碳源。
Nutrient metabolism is regulated for adaptation to, for example, environmental alterations, cellular stress, cell cycle, and cellular ageing. This regulatory network consists of cross-talk between cytoplasmic organelles and the nucleus. The ras-like nuclear small G protein, Ran, functions in nuclear-cytosolic transport and regulatory signal transmission. In yeast, some genes involved in the Ran system in yeast are required for growth on glycerol medium. Growth deficiency, due to mutations in theGSP1gene, which encodes Ran, is allele specific. Specifically in this study, thegsp1-1894cells lost mitochondria, and could not grow on media containing glycerol, galactose or maltose. However, thegsp1-1894cells grew better on a high salt medium (1 M NaCl) and had increased expression levels ofGPD1-lacZ.Furthermore, disruption of theHOG1gene suppressed their growth deficiency on glycerol medium. These findings suggest that altered activation of Hog1 in thegsp1-1894cells resulted in the loss of mitochondria and inhibition of glycerol metabolism. Growth deficiency of thegsp1-1894cells on galactose medium was further suppressed by high dosage of theSIP2DNA, which encodes the cytosolic β subunit of AMPK. This suggests that higher cytosolic activity of AMPK is required for the utilization of an alternative carbon source ingsp1-1894cells.