A novel microstep device for the size separation of cells
A novel microstep device for the size separation of cells
复制标题
一种用于细胞尺寸分离的新型微步装置
作者：
Sarah Vankrunkelsven;D. Clicq;K. Pappaert;W. Ranson;C. De Tandt;H. Ottevaere;H. Thienpont;G. Baron;G. Desmet
我们报告了一系列初步实验，研究了一种新方法对纳米和微米级颗粒和细胞的尺寸分离的适用性。其工作原理是基于剪切驱动流通过阶梯式锥形微通道或纳米通道的应用。对0.5 μ m和1.0 μm羧化聚苯乙烯珠粒混合物以及金黄色葡萄球菌和酿酒酵母细胞的二元混合物和S.酿酒酵母和大肠杆菌细胞。
We report on a series of preliminary experiments investigating the applicability of a novel method for the size separation of nano‐ and microsized particles and cells. The working principle is based on the application of a shear‐driven flow through stepwise tapered micro‐ or nanochannels. Size separations of mixtures of 0.5 and 1.0 μm carboxylated polystyrene beads as well as of binary mixtures of Staphylococcus aureus and Saccharomyces cerevisiae cells and of S. cerevisiae and Escherichia coli cells are demonstrated.