Proposed Protocol for Human Hair Cortisol Extraction without Pulverization
Proposed Protocol for Human Hair Cortisol Extraction without Pulverization
复制标题
无需粉碎即可提取人发皮质醇的拟议方案
DOI：
10.11239/jsmbe.55.205
复制
发表时间：
2017
影响因子：
--
通讯作者：
野村 収作
中科院分区：
文献类型：
--
作者：
吉田 怜楠;野村 収作
由于心理压力而释放到血液中的皮质醇，并在头发生长过程中积聚在头发中。因此，人类毛发皮质醇（HHC）可以被认为是一种回顾性和长期的压力指数。然而，HHC测定方案有一些技术问题需要解决。其中一个问题是HHC提取过程中头发样品的过度粉碎会降低提取效率。在本研究中，我们研究了头发粉碎和提取时间对HHC提取的影响。从20名年轻参与者中提取HHC，使用各种机械样品分解方案（使用粉碎机粉碎，使用剪刀破碎，未经处理的1厘米头发片段）和提取时间（1,6,24,48和72小时）。结果表明，提取时间越长，HHC的提取率越高。值得注意的是，无论何种粉碎方式，48 h后HHC的提取率均达到峰值。此外，当提取时间为48 h或更长时，不同分解程序的方案提取的HHC量高度相关。因此，我们的结果表明，当提取时间超过48 h时，HHC可以按照不粉碎的协议重复纯化。
Cortisol that is released into the blood stream in response to psychological stress and accumulates in human hair during its growth. Thus, human hair cortisol (HHC) can be considered a retrospective and long-term index of stress. However, the protocol for HHC determination has technical issues that have to be resolved. One of the issues is that excessive pulverization of hair samples during HHC extraction can reduce extraction efficiency. In the present study, we investigated the effects of hair pulverization and extraction time on HHC extraction. From 20 young participants, HHC was extracted using various protocols for mechanical sample decomposition (powdered using a pulverizer, fragmented using scissors, and untreated 1-cm hair segment) and extraction times (1, 6, 24, 48, and 72 h). We showed that increasing the extraction time increased the amount of HHC extracted. Notably, after 48 h, the amount of HHC extracted peaked regardless of the type of pulverization. Moreover, high correlation of the extracted HHC amount was observed for protocols involving different decomposition procedures when the extraction time was 48 h or longer. Thus, our results indicate that HHC can be purified reproducibly following a protocol without pulverization when the extraction period is over 48 h.