A rat model for functional characterization of pregnancy-induced denervation and postpartum reinnervation in the myometrium and cervix: a superfusion study.
A rat model for functional characterization of pregnancy-induced denervation and postpartum reinnervation in the myometrium and cervix: a superfusion study.
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用于妊娠诱导的子宫肌层和子宫颈去神经支配和产后神经支配功能表征的大鼠模型：一项灌注研究。
作者：
I. Zupkó;D. Csonka;G. Falkay
妊娠引起的支配子宫的肾上腺素能神经的快速变性是一个众所周知但知之甚少的现象。由于大多数已发表的调查进行了组织学分析，我们的目的是描述在怀孕期间的肾上腺素能功能的损失和在产后期间的重新神经支配的程序。将大鼠子宫肌层和宫颈样本加载[3H]去甲肾上腺素，然后转移至灌流室中。洗脱期后，收集级分。用电场刺激第五和第十五级分组织。测定各组分的[3H]去甲肾上腺素含量以及组织中的残留量。子宫肌层[3H]去甲肾上腺素释放在妊娠早期显著减少，在妊娠晚期不存在。在植入部位和植入区域之间检测到释放曲线的差异。作为一个细化的结果，以前的组织化学研究，去甲肾上腺素能功能的子宫颈被发现在产后早期深受影响。妊娠诱导的去神经程序可以通过[3H]去甲肾上腺素负荷后的超融合技术进行。由于我们的技术被认为是相似的组织学方法的敏感性，灌流可以被视为妊娠诱导的去神经支配的功能研究的模型。
The pregnancy-induced rapid degeneration of the adrenergic nerves innervating the uterus is a well-known but poorly understood phenomenon. Since most of the published investigations were carried out by histological assay, our aim was to describe the loss of the adrenergic function during pregnancy and the re-innervational procedure in the postpartum period. Myometrial and cervical samples from rats were loaded with [3H]noradrenaline and then transferred into a chamber for superfusion. After a wash-out period, fractions were collected. The fifth and fifteenth fraction tissues were stimulated with an electric field. The [3H]noradrenaline contents of the fractions were determined, together with the amount remaining in the tissue. The myometrial [3H]noradrenaline release was substantially decreased in early pregnancy, and absent in the late stage. Differences in release profile were detected between the implantation sites and the interimplantation areas. As a refinement of the results of previous histochemical studies, the noradrenergic functions of the cervix were found to be deeply affected in the early postpartum period. The pregnancy-induced denervational procedure can be followed by means of a superfusional technique after [3H]noradrenaline loading. As our technique is considered to be similar in sensitivity to histological methods, superfusion can be regarded as a model for functional investigations of pregnancy-induced denervation.