Lidocaine inhibits granulocyte adherence and prevents granulocyte delivery to inflammatory sites.
Lidocaine inhibits granulocyte adherence and prevents granulocyte delivery to inflammatory sites.
复制标题
利多卡因抑制粒细胞粘附并防止粒细胞输送至炎症部位。
作者：
R. Macgregor;R. Thorner;D. M. Wright
将全血或 PMN 在盐水中与 1O 2 或 1O 摩尔浓度的利多卡因一起孵育，会导致粒细胞粘附的可逆抑制。静脉注射单次推注剂量的利多卡因 2.5 mg/kg，在 5 mm 处可抑制兔子的依从性达 55.4%，给药后 1·5 mm 时恢复正常。当推注剂量后输注利多卡因时。 0.3 mg/kg/mm，输注期间的依从性保持在对照值的 50% 以下。给予利多卡因的动物血浆抑制正常粒细胞的粘附。将血浆用改良汉克斯溶液透析24小时，即可完全去除抑制因子。利多卡因输注可防止 PMN 输送至炎症部位：接受无菌性腹膜炎的正常兔子在 6 小时内出现渗出物，平均含有 19.280 PMN/立方毫米，而输注利多卡因的兔子为 388/立方毫米（对照的 2%）。相比之下。经甲泼尼龙治疗的动物出现渗出物 PMN 计数为对照组的 40%。当葡萄球菌引起腹膜炎时，6只注射利多卡因的动物中有5只死亡，而6只未注射利多卡因的动物中只有1只死亡。对实验感染皮肤的病理检查表明，对照动物在皮内注射活葡萄球菌后 1-2 小时出现以水肿和明显 PMN 浸润为特征的炎症反应。相比之下，注射利多卡因的动物的活检仅显示水肿，几乎没有中性粒细胞浸润。因此，利多卡因在体外和体内抑制粒细胞粘附，并显着抑制中性粒细胞向炎症部位的递送。
Incubation of whole blood or PMNs in saline with 1O 2 or 1O molar concentrations of lidocaine causes a reversible inhibition of granulocyte adherence. A single bolus dose of lidocaine, 2.5 mg/kg, given intravenously inhibits adherence in rabbits by 55.4% at 5 mm, with return to normal by 1 5 mm after dose. When the bolus dose is followed by an infusion of lidocaine. 0.3 mg/kg/mm, adherence stays at less than 50% of control values for the duration of the infusion. Plasma from animals given lidocaine inhibits adherence of normal granulocytes. and the inhibiting factor is removed completely by dialysis of the plasma against modified Hanks’ solution for 24 hr. Lidocaine infusion prevents delivery of PMNs to sites of inflammation: normal rabbits given sterile peritonitis developed exudates in 6 hr containing a mean of 19.280 PMN/cu mm compared to 388/cu mm in lidocaine-infused rabbits (2% of control). By comparison. methylprednisolone-treated animals developed exudate PMN counts 40% of control. When peritonitis was induced by staphylococci, 5 of 6 lidocaine-infused animals died, whereas only 1 of 6 noninfused animals died. Pathologic examination of experimentally infected skin showed that control animals developed an inflammatory reaction characterized by edema and pronounced PMN infiltration 1 2 hr after intracutaneous injection of live staphylococci. In contrast, biopsies from lidocaine-infused animals showed only edema, with virtually no PMN infiltration. Thus, lidocaine inhibits granulocyte adherence in vitro and in vivo and markedly suppresses delivery of PMNs to sites of inflammation.