Cryogenic Ion Spectroscopy of the Green Fluorescent Protein Chromophore in Vacuo
Cryogenic Ion Spectroscopy of the Green Fluorescent Protein Chromophore in Vacuo
复制标题
真空中绿色荧光蛋白发色团的低温离子光谱
DOI：
10.1021/acs.jpclett.9b02916
复制
发表时间：
2019
期刊：
影响因子：
--
通讯作者：
Weber, J. Mathias
中科院分区：
文献类型：
--
作者：
Zagorec-Marks, Wyatt;Foreman, Madison M.;Verlet, Jan R.;Weber, J. Mathias
我们展示了真空低温下绿色荧光蛋白发色团阴离子模型的 S1← S0 跃迁光谱，显示了以前未解决的振动特征，并以前所未有的精度解析了 20930 cm–1（477.8 nm）处的带起源。振动光谱确定该分子在低温下处于Z异构体中。在温度升高时，S1←S0带转变为红色，我们暂时将其归因于E异构体的新兴群体。
We present the spectrum of the S1← S0transition of an anionic model for the chromophore of the green fluorescent proteinin vacuoat cryogenic temperatures, showing previously unresolved vibrational features, and resolving the band origin at 20 930 cm–1(477.8 nm) with unprecedented accuracy. The vibrational spectrum establishes that the molecule is in theZisomer at low temperature. At increased temperature, the S1← S0band shifts to the red, which we tentatively attribute to emergent population of theEisomer.